10 Questions 180 Answers 0 Followers
Questions related from Sven Dittmann
I have a qPCR-question. Maybe this is a trivial question. When we normally validated new primer, we make a dilution series from 1:10, 1:100, 1:1000…. Now our gene of interest is not highly...
05 May 2019 4,418 6 View
Hello, I have the following question. Does anyone know a way that Pubmed IDs in Microsoft Word are automatically converted into citations WITHOUT they are previously imported, for example, at...
11 November 2018 4,647 1 View
I asked for a colleague of my. She wants to remove a N-glycosylation site with mutagenesis. Now she read many literature as she told me and now wonders why researchers only mutated this sites...
07 July 2013 8,567 7 View
I ask for a colleague and hope you can help her. She overexpressed a human protein in a bacterium and then she made a shedding experiment (enzyme which cleaves the protein). Now she wants to...
07 July 2013 9,637 23 View
Does anybody know a method to differentiate between cells before and after cell division? We plan a migration assay and therefore we plan the following project: 1. First we seed out HUVEC cells...
07 July 2013 6,396 11 View
A colleague prepared Western blot from a protein which should normally appear only as one band on the Western blot Membrane. The concentration of the protein is low, BCA test negative. What she...
05 May 2013 6,562 11 View
I tried to research the halflife of a protein. This surface protein is expressed continuously by cells. So an idea is the biotinylation of the protein in cell culture. I am absolutely new in this...
11 November 2012 8,812 13 View
At the moment, I treat cells with a drug to test the effect of the drug for an expression profile of transcription factors. In low concentrations of the drug, the expression of the transcription...
11 November 2012 9,362 9 View
Hello, I have A549 cells in culture. Normally they attached really nice on normal flask, T25 or T75 without coating the flask. Now I freeze some of these cells and after thawing they don't...
07 July 2012 7,179 9 View
07 July 2012 1,533 5 View
