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Questions related from Alasdair Russell
I am growing cells in 96 well plates and then essentially performing a western blot in the plate. This involves (among other things): - Fixing (4% paraformaldehyde/10 min) - Permeabilising...
02 February 2017 4,210 4 View
Hi All A quick series of questions about packaging rAAV: I want to produce AAV vectors, however I'm having difficulty finding the helper plasmids to package them. Any ideas where to look (I...
05 May 2016 6,655 1 View
Hi all I'm looking for a range of reagents (eg Nocodazole) or methods (eg Mitotic shake off) to block/stall cell lines grown in culture in G2-M phases of the cell cycle. Ideally I'd want to block...
01 January 2016 9,735 2 View
I have made an array of synthetic tumours. I did this by taking FACS sorted pure populations of primary normal epithelial cells and transforming them using a combination of 3 different...
05 May 2013 8,825 12 View
I'm hoping that one of you clever and wonderful people knows of a software solution that will allow me to project manage my Postdoc. I am looking for something that has some sort of graphical...
01 January 2013 7,326 1 View
We have infected both primary mouse stem and progenitor cells and primary human stem and progenitor cells with Sigma Aldrich’s VSV-G pseudotyped lentivirus to great success. Does anyone know if it...
10 October 2012 6,906 2 View
Hi, I wonder if anyone can give me some tips. I'm a cell biologist, and a complete Bioinformatics noob. I am growing some breast cancer cells that have a fluorescent protein in them that will...
05 May 2012 9,437 1 View