Alasdair Russell

7 Questions 64 Answers 0 Followers

Questions related from Alasdair Russell

Simple DNA extraction protocol from Formaldehyde fixed cells in a 96 well plate?

I am growing cells in 96 well plates and then essentially performing a western blot in the plate. This involves (among other things): - Fixing (4% paraformaldehyde/10 min) - Permeabilising...

02 February 2017 4,201 4 View

rAAV packaging plasmids... which ones? and where to get them from?

Hi All A quick series of questions about packaging rAAV: I want to produce AAV vectors, however I'm having difficulty finding the helper plasmids to package them. Any ideas where to look (I...

05 May 2016 6,643 1 View

Reversible drugs/approaches that stall cell cycle progression in G2-M phase?

Hi all I'm looking for a range of reagents (eg Nocodazole) or methods (eg Mitotic shake off) to block/stall cell lines grown in culture in G2-M phases of the cell cycle. Ideally I'd want to block...

01 January 2016 9,718 2 View

How to determine clonality of a tumour by lentiviral integration sites?

I have made an array of synthetic tumours. I did this by taking FACS sorted pure populations of primary normal epithelial cells and transforming them using a combination of 3 different...

05 May 2013 8,813 12 View

Software suggestions for managing experimental projects?

I'm hoping that one of you clever and wonderful people knows of a software solution that will allow me to project manage my Postdoc. I am looking for something that has some sort of graphical...

01 January 2013 7,315 1 View

Is it possible to infect rat primary neurons with lentivirus?

We have infected both primary mouse stem and progenitor cells and primary human stem and progenitor cells with Sigma Aldrich’s VSV-G pseudotyped lentivirus to great success. Does anyone know if it...

10 October 2012 6,890 2 View

How do I track fluorescent single cells dividing in a petrie dish over time to determine hierarchical relationships based on their fluorescence?

Hi, I wonder if anyone can give me some tips. I'm a cell biologist, and a complete Bioinformatics noob. I am growing some breast cancer cells that have a fluorescent protein in them that will...

05 May 2012 9,423 1 View