Hossam Murad

7 Questions 21 Answers 0 Followers

Questions related from Hossam Murad

Cloning vector for high GC ratio gene? - is GC ratio a criteria in choosing vector?

I'm trying to clone a ~900 bp gene which has a high GC ratio (about 75%) in E. coli. (Top10). The thing is, I'm not sure if the ratio of GC is a criteria in choosing the vector or rather, if...

05 May 2016 7,271 7 View

Cloning vector for high GC ratio gene? - is GC ratio a criteria in choosing vector??

I'm trying to express a my target gene which has a high GC ratio about 75% in E. coli. The thing is, I'm not sure if the ratio of GC is a criteria in choosing the vector or rather, if there are...

05 May 2016 1,121 1 View

Why is there an up-regulation on DNMT1, DNMT3a,DNMT3b gene expression after gamma irradiation?

I treated an Astrocytoma cell line to 10 gry of (gamma Irradiation), I noticed that an up-regulation of DNMT1, DNMT3a and DNMT3b gene expression. is there any explanation?

08 August 2015 4,207 5 View

How can I detect the membrane receptor of MDA-MB-231 for my extract?

I have a red seaweed extract, so I want to know which membrane receptor binds to my extract to activate the pathway?

05 May 2013 2,570 4 View

Full length NS5B gene amplification in HCV-4

I work with HCV-4, and I want to amplify the full lengh of the NS5B gene for the HCV4 type. Could anyone share the primer sequences and the PCR program?

06 June 2012 2,726 8 View

Do you know a target gene for growth hormone in human cell line?

I want to know the effects of growth hormone on human cell lines

05 May 2012 2,979 6 View

How I can create cancer of the lung of liver in rat?

I want to use the special radioactive element to detect a lung or liver cancer

04 April 2012 4,272 5 View