Dipon Das

8 Questions 94 Answers 0 Followers

Questions related from Dipon Das

Does anyone know a protocol for gene overexpression in a bacterial strain?

I have a gene cloned in a CMV mammalian vector, transfected into bacterial strain DH5 alpha. I am unable to induce the expression of the plasmid even on adding IPTG (varying concentration) and...

01 January 2013 3,795 8 View

What are the experimental modes of determining the interaction of a protein and a ligand?

What are the experimental modes of determining the interaction of a protein and a ligand (small molecule)?

11 November 2012 530 11 View

Can XRD or any other biophysical methods other than ITC be used to study the interaction between a protein and a small ligand molecule ?

11 November 2012 5,888 5 View

Is it possible to determine a protein as a cancer biomarker using only cell lines?

Is it possible to determine a protein as cancer biomarker using only cell lines? If yes, what are the methods should be employed to know that the protein is a biomarker?

07 July 2012 7,583 19 View

I have a novel bacterial protein, which while isolating, doesn't come out in the supernatent as it forms inclusion bodies, thereby making it impossibl...

I have a novel bacterial protein, which while isolating, doesn't come out in the supernatent as it forms inclusion bodies, thereby making it impossible to crystallize. Can anyone suggest what I...

05 May 2012 6,553 6 View

Virtual screening

Can anyone suggest free web software with which I can do virtual screening against some free compound database like ZINC using a protein structure ?

05 May 2012 260 11 View

Anti- cancer drugs

What can be the main reason for an anti-cancer drug being effective in some individuals and bein ineffective in other individuals???

04 April 2011 8,897 40 View

Cancer cell property

Why does cancer cells tend to become drug resistance??

01 January 2011 4,195 4 View