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Questions related from Thang Luong Pham
Dear friends, I am working on RPE (human/mice primary RPE and ARPE19) for a while. The seeded density is 100.000 cells/ml. For T-75 flask, 6-well plate I used 20 ml and 3 ml/well respectively. The...
01 January 2015 670 0 View
We know that chemicals which can attach to DNA structure are toxic, although toxicity is very low. Therefore, I guess that some manufacturers give us inaccurate information when they says that...
01 January 2014 9,235 2 View
I am expressing some proteins and studying about interactions of our interested proteins. For that reason, I want to evaluate binding abitlity between protein-protein via a significant values– Ka...
05 May 2013 6,662 4 View
I am conducting multiplex PCR experiments for infertility detection. And I want to collect DNA from blood sample. However, I do not what the best method for DNA extraction is. What is your experience?
03 March 2013 1,145 16 View
There are many protocols with a lot of adjuvants such as aluminium salts, squalene, freunds, etc. Can you give me your experience with how to choose the most comfortable adjuvant for total and...
01 January 2013 7,067 8 View
I am going to conduct ELISA assay, but I do not know what microtitration plate is best for high efficiency of protein fixation.
01 January 2013 5,906 12 View
There are many way arrange exon in the alternative slicing, so how to identify what exon and exon's order in the mature mRNA ???
12 December 2012 822 2 View
I am a master student and need to learn about choosing the right cloning strategy. Therefore, I really want to read more books about that issue. Any suggestions?
11 November 2012 9,720 3 View
I am need to buy a new UV transilluminator. A company presented a new LED - UV transilluminator to me and I am interested in buying it, but don't have any experience with this instrument. Can you...
11 November 2012 7,872 4 View