Thang Luong Pham

9 Questions 28 Answers 0 Followers

Questions related from Thang Luong Pham

What is the best concentration for initiating the ARPE19 / RPE in T-flask and N-well plate with DMEM-F12?

Dear friends, I am working on RPE (human/mice primary RPE and ARPE19) for a while. The seeded density is 100.000 cells/ml. For T-75 flask, 6-well plate I used 20 ml and 3 ml/well respectively. The...

01 January 2015 672 0 View

Some chemical for DNA staining, such as SYBR non-toxic for scientists?

We know that chemicals which can attach to DNA structure are toxic, although toxicity is very low. Therefore, I guess that some manufacturers give us inaccurate information when they says that...

01 January 2014 9,237 2 View

What is the best method to identify Kd (dissociation constant) and Ka (association or equilibrium constant)?

I am expressing some proteins and studying about interactions of our interested proteins. For that reason, I want to evaluate binding abitlity between protein-protein via a significant values– Ka...

05 May 2013 6,663 4 View

What is the best kit or protocol for DNA genome extraction from blood sample?

I am conducting multiplex PCR experiments for infertility detection. And I want to collect DNA from blood sample. However, I do not what the best method for DNA extraction is. What is your experience?

03 March 2013 1,146 16 View

What is the best immunologic adjuvant for mice and rabbits?

There are many protocols with a lot of adjuvants such as aluminium salts, squalene, freunds, etc. Can you give me your experience with how to choose the most comfortable adjuvant for total and...

01 January 2013 7,068 8 View

What is the best microtitrate plate for direct-ELISA technique?

I am going to conduct ELISA assay, but I do not know what microtitration plate is best for high efficiency of protein fixation.

01 January 2013 5,906 12 View

What is the best technique to identify alternative slicing result in vivo and in vitro ?

There are many way arrange exon in the alternative slicing, so how to identify what exon and exon's order in the mature mRNA ???

12 December 2012 823 2 View

Do you have any e-books on cloning guidelines for selecting restriction enzymes when RE sites are located on both the target gene and Multi Cloning Site?

I am a master student and need to learn about choosing the right cloning strategy. Therefore, I really want to read more books about that issue. Any suggestions?

11 November 2012 9,721 3 View

Do you have any advice on LED - UV transilluminator?

I am need to buy a new UV transilluminator. A company presented a new LED - UV transilluminator to me and I am interested in buying it, but don't have any experience with this instrument. Can you...

11 November 2012 7,873 4 View