Matthew Lakins

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Questions related from Matthew Lakins

Has anyone used the tumour cell line CT26.CL25?

CT26.CL25 is a carcinoma cell line derived from colon. This cell line has been stably transduced wtih the lacZ gene encoding the model tumor associated antigen (TAA), beta-galactosidase (beta-gal)...

09 September 2016 2,570 1 View

Is there a cell line that expresses membrane-bound CD40L?

I know of the 3T3-mCD40L cell line that expresses a soluble form, but how about a good cell line that expresses CD40L that remains surface bound?Ideally I would like the ligand immobilised on...

07 July 2015 576 2 View

What's the best way to extract RNA from small numbers of FACS sorted cells?

I am having a bit of trouble trying to obtain good quality and decent amounts of mRNA from ~5,000-10,000 FACS sorted cells. Tumours are being dissociated to a single cell suspension, stained for...

07 July 2013 3,268 18 View

How can I confirm the PC-3 cell line I have been working on is actually a PC-3 cell line?

You read about how people realise too late that their cell line has been contaminated by, for example, HeLa cells which outgrow and overtake the original cell line. So what is the best way of...

11 November 2012 9,394 12 View

How to identify membrane-bound compartments?

I've been imaging fibroblasts 'eating'/engulfing particles and they seem to be stored/held within small spherical structures surrounding the nucleus. How can I best identify what these...

11 November 2012 8,139 6 View