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Questions related from Matthew Lakins
CT26.CL25 is a carcinoma cell line derived from colon. This cell line has been stably transduced wtih the lacZ gene encoding the model tumor associated antigen (TAA), beta-galactosidase (beta-gal)...
09 September 2016 2,584 1 View
I know of the 3T3-mCD40L cell line that expresses a soluble form, but how about a good cell line that expresses CD40L that remains surface bound?Ideally I would like the ligand immobilised on...
07 July 2015 590 2 View
I am having a bit of trouble trying to obtain good quality and decent amounts of mRNA from ~5,000-10,000 FACS sorted cells. Tumours are being dissociated to a single cell suspension, stained for...
07 July 2013 3,278 18 View
You read about how people realise too late that their cell line has been contaminated by, for example, HeLa cells which outgrow and overtake the original cell line. So what is the best way of...
11 November 2012 9,407 12 View
I've been imaging fibroblasts 'eating'/engulfing particles and they seem to be stored/held within small spherical structures surrounding the nucleus. How can I best identify what these...
11 November 2012 8,152 6 View