Varshiesh Raina

3 Questions 2 Answers 0 Followers

Questions related from Varshiesh Raina

Which is the best transfection reagent to transfect siRNA in SHSY5Y cells?

I have been trying to transfect siRNA in SHSY5Y cells using lipofectamine 2000 but could not find knockdown of my protein of interest.

02 February 2013 5,326 1 View

What is the best and easiest way of inducing T Cell activation in-vivo?

I intend to study a protein (X) in context to its role during T Cell activation. It doesn't matter whether it is Th1 or Th2. I need some suggestions like the kind of mitogen, chemically induced etc.

11 November 2012 666 7 View

Can anyone recommend a protocol for nuclear protein isolation from neurons?

I want to isolate a good quality of protein from neuronal cell lines. I want to know about the effective lysis buffer that does not give any artifacts.

11 November 2012 1,460 1 View