Robert Maughan

4 Questions 5 Answers 0 Followers

Questions related from Robert Maughan

Can lysates prepared using a detergent based lysis method be used in cholesterol quantitation?

I am planning experiment in which I would like to use detergent based lysis to prepare cellular lysates for cholesterol quantiation by the cholesterol oxidase method. I am aware that endogenous...

06 June 2016 7,975 1 View

How do you optimise efficiency and primer concentration for low abundant transcripts?

I'm trying to measure the efficiency and optimise the primer concentration for my assay which is measuring TBP expression. I'm doing this with a standard curve of cDNA with the points: 100ng,...

04 April 2014 5,492 0 View

What is the lowest acceptable CT/CP in a QPCR experiment?

I have read and heard quite a bit that the lowest admissable CT/CP value is ~12-15. My idea for the basic logic of this is that the reaction is likely to have been overloaded with initial...

03 March 2014 7,709 7 View

Use of vancomycin for primary human adipocyte cultures?

Our lab has recently started culturing cells from elective abdominal surgery adipose tissue biopsies. We have had some difficulty regarding contaminated cultures and we suspect that there may be...

09 September 2012 646 3 View