Is there a way to measure alpha-haemolysin production in Escherichia coli?
Preferently a phenotypic method rather than quantyfing hlya gene expression.
The phenotypic quantification of alpha-haemolysin production in uropathogenic E. coli can be accomplished through the blood agar plate method. In this technique, bacterial cultures are spot-inoculated onto 5% sheep blood agar plates and incubated at 37°C for 24-48 hours. The alpha-haemolysin production is quantified by measuring the diameter of the clear zone of complete haemolysis (beta-hemolysis) around the bacterial colonies. A larger zone of hemolysis indicates higher alpha-haemolysin production. This method can be made semi-quantitative by establishing a standardized scoring system based on the diameter of hemolytic zones, typically categorizing them as strong (>5mm), moderate (2-5mm), or weak (
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