Tetra-ARMs PCR setup?

Zahra Taherian-Esfahani @Zahra-Taherian-Esfahani

21 November 2024 1 3K Report

Hello everybody

I sent a question about Tetra-ARMs PCR a few minutes ago .i sent it suddenly y some spelling errors. I apologize you .i am sending the corrected question .

I want to setup Tetra-ARMs PCR . mutant allele band is very weak in spite of decreasing in outer primers concentration ?what can i do?

Paul Rutland

You can try increasing the amount of primer for the mutant allele and also slightly increasing the Mg concentration and lowering the annealing temperature if that does not lead to non specificity of the other primers. You might want to check the primer design using the

https://snp.biotech.edu.lk/arms.php tool

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