Discuss everything related to viruses | Contact experts in Virology to get answers
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Questions related to Virology
Are these structures part of the Golgi membrane itself? or are they independent viral structures?
13 September 2015 9,821 1 View
Hi everyone, I am searching information for any virus member in Flaviviridae family which dose not create replication vesicles in ER membrane during their infection cycle.If you know any kind of...
10 September 2015 10,040 3 View
09 September 2015 1,483 1 View
I need to carry out the titration of the dengue virus by focus forming units (FFU). Does anyone know of someone article and which the formula to determine the viral titer?
09 September 2015 3,307 1 View
09 September 2015 3,566 3 View
The main bee viruses are classified in two families. The Iflaviridae that hosts the deformed wing virus (DWV), the slow bee paralysis virus (SBPV) and the sac brood virus (SBV). the...
31 August 2015 5,132 3 View
08 August 2015 5,598 3 View
I am working on virotherapy using adenovirus.I want to return E1A adenovirus (including promoter + CDS) to pad/pl/dest vector (invitrogen).If I return E1A gene (from nucleotide 400 to 1700 of wild...
08 August 2015 7,286 1 View
what is your opinion about best moi OF ADENOVIRUS for transfection of MCF-7 cell?
08 August 2015 9,320 2 View
I am measuring viral concentration in groundwater samples with high and low ionic strength, DOM and SOM. Results show that samples with no SOM have the opposite relationship with ionic strength...
08 August 2015 6,674 1 View
Hi! I'm starting to standardize the PCR, so I'm not really sure wich is the best DNA concentration or primers concentration that I need to use Mzia 1(5' GATATTTGIGGIGTTCAGCCIATGA 3') Mzia 2(5'...
15 July 2015 8,458 5 View
07 July 2015 10,003 7 View
Hi Guys I`m in need of your opinions on interpretation of HI resuts I`ve attached a couple of annotated plates where I seem to have four different outcomes. Could you read them and say if you...
20 June 2015 3,874 8 View
Hi guys I'm running the HI assay on 2300 sera using A/California/07/09pdm as the antigen and type0 human rbcs. All sera have been rde treatedbut is itnecessary torrent all by adsorptionwith rbcs...
16 June 2015 6,260 2 View
06 June 2015 9,225 2 View
06 June 2015 654 9 View
Hi everyone, Currently, I am designing primers for the real-time analysis. I have observed in the nucleoprotein and PB2 of the influenza virus that a huge amount of variation is consistently...
06 June 2015 5,765 5 View
I am detecting PRRS virus in lung samples using a diagnostic PRRS virus assay which is very reliable, it targets ORF 7 and amplifies a 80bp product. With any positive samples I detect, I am then...
06 June 2015 6,167 2 View
I have a virus isolated in cell culture. I am going to extract virus RNA and want to get rid of cellular RNA before sharing sample to NGS facility. Anyone who can respond or guide, please?
05 May 2015 4,244 13 View
PRRSV infects macrophages and it found in pulmonary alveolar macrophages in lungs. And Macrophages presents in blood cells. The RNA isolated from white blood cells contains PRRSV RNA. And Is...
05 May 2015 8,057 4 View
I need a review study on lumpy skin disease.
26 April 2015 9,949 2 View
I am currently doing some virus infectious clones work.I have tried to in-vitro transcribe the coxsackievirus A16 (picornavirus) infectious clone to make rna, and then transfect them into HEK 293...
24 April 2015 8,725 8 View
Identification of Snidbis and Chikungunya Viruses We Need positive control for RT-PCR's of Sindbis and Chikungunya viruses , could anyone kindly provide them , they could be plasmid/s containing...
04 April 2015 3,030 3 View
04 April 2015 2,323 1 View