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Questions related to Virology
For virus isolation from raw fruit samples, the solution after precipitating with Polyethylene Glycol 8000 is becoming very thick and so it is not being feasible to use it for injection filtration...
28 March 2016 2,593 1 View
Is it true that viruses don't have colors or our current methods are limited to detect it?
12 March 2016 2,704 8 View
Foot & Mouth Disease Control Programme was launched with great promise more than a decade ago. But where is control, disease has haunted several time during the period even the herds at the...
03 March 2016 7,732 1 View
PR8 and A549
03 March 2016 970 0 View
03 March 2016 3,069 1 View
Foot and Mouth Disease Control Programme (FMD-CP) in India started more than a decade ago (2003), in UP (2nd phase) 18th round of Compulsory Vaccination (twice in a year) is over, but with no...
03 March 2016 10,262 2 View
Dear All, I have been trying to generate retrovirus using 293T and Lipofectamine 2000. But, so far, I don't get detectable virus. I am using pBABE retroviral vector and pCLAmpho expressing...
03 March 2016 7,903 1 View
03 March 2016 4,608 9 View
Virology, Nature
03 March 2016 4,076 1 View
For sf9 vector transformation, almost all literature suggests to transform it when the cells is in adherent form. my cells are suspension in grace insect medium supplemented. Would you please...
03 February 2016 9,861 4 View
02 February 2016 1,658 5 View
I have some technical questions, I would really appreciate some feedback.
02 February 2016 6,106 4 View
It is a problem with rabies virus, which does not propagate in BHK cells. The first and second passage after the transfer from NAC1300 is good, but in the next passages level of growth is falling....
02 February 2016 7,148 8 View
I'm looking for published or unpublished papers.
02 February 2016 480 8 View
I have been using Tk- cells for vaccinia virus infection. They were working fine till all of a sudden, shortly after adding the virus they lift off. I tried everything including changing the PBS...
02 February 2016 1,182 2 View
Hi All, I am currently starting to think about some future independent research projects that related to virology. I know which general aspect I want to study about virus; however, I can't decide...
02 February 2016 6,218 6 View
I would like to now mechanism by the Zika virus produces the symptoms in humans, especially the microcephaly and Guillain–Barré syndrome (GBS).
02 February 2016 2,099 11 View
Each cell line would express one of the nucleoporins fused to GFP.
02 February 2016 8,087 3 View
02 February 2016 8,022 3 View
I infected human neural stem cells with a RFP tagged viral construct, and wanted to sort infected and undifferentiated cells. For this I use two intracellular markers, Sox2 and Nestin. When I ran...
21 January 2016 8,283 6 View
This question came up after reading the paper by Engelhardt et al., 2015.
01 January 2016 2,819 1 View
01 January 2016 6,888 7 View
I'm now investigating the role of c-jun and c-fos in mouse. Therefore, I need c-jun and c-fos expressing lenti- or adenovirus to infect quiescent cells. Also, I need some c-jun and c-fos shRNA...
01 January 2016 4,132 1 View
If I would like to study the release of inactivated polio virus, how could separate the released polio virus from the carrier? It seem the size of the polio virus is too large for dialysis based...
01 January 2016 8,534 1 View