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Questions related to Virology
Dear colleagues, I address to the experts on how to calculate the antiviral activity of compounds in vitro. I always thought that the medium effective conc. of a compound (EC50) – it’s the conc....
12 December 2014 1,893 3 View
I am going through my first course in Virology and already I am aware of the enormous amount of subfields within virology. But I know that I must establish some sort of "base" knowledge before I...
12 December 2014 6,689 3 View
I learned that people normally use 293T or 293FT to package HIV-1 and then infect TZM-bl. Can HIV-1 get released from TZM-bl and then infect other TZM-bl cells? I read about using pseudo virus to...
12 December 2014 833 19 View
What is composition of the most external layer of the capsid? Of course it could depend on the phage type. However, does someone know if there are specific studies with a given phage or group of...
12 December 2014 447 2 View
02 December 2014 4,107 18 View
Hello I am trying to find a method to inactivate swine influenza viruses that are produced in eggs, most of the items gives formalin, but I find a specific protocol, I need suggestions and ideas...
28 November 2014 4,577 16 View
I want to induce the lytic cycle of EBV in Akata and Jijoye cell lines using TPA (tetradecanoyl phorbol acetate), sodium butyrate and IgG. I was wondering how long it takes before the induction is...
15 November 2014 8,796 7 View
siRNA could lead to the degradation of a target mRNA, but I could only understand in the case that this target mRNA synthesized by the host cell itself; thus, how can siRNA act as a natural...
14 November 2014 6,336 5 View
11 November 2014 7,873 17 View
11 November 2014 9,396 6 View
As viruses become day to day smarter and develop resistance by modifying his own gentic makeup to most of the fixed dose molecules. Is it possible to treat such viruses cases with varying degree...
11 November 2014 5,616 3 View
If I have a brand new virus or phage's genome sequence, which have few similarity with known genes. How to predict its host based on its genome? Or is there any other technology to do this?
11 November 2014 1,281 8 View
What are the glycosylation site prediction servers? I want to use on viral (human host) proteins, since viral proteins use host glycosylation machinery. Please suggest.
11 November 2014 1,888 6 View
For extending a part of research, we need to have culture of phocine distemper virus. Can anyone here guide us how we can provide that?
11 November 2014 7,554 1 View
11 November 2014 5,354 7 View
I was just wondering if one could resuspend the lentivirus pellet in whatever culture medium, instead of PBS. In this way maybe the final concentration of virus can get higher when later...
27 October 2014 9,059 7 View
Detection of EBV-encoded RNA (EBER) via in situ hybridization is the gold standard to determine whether cells contain EBV. I was wondering however whether in EBER-negative cells EBV episomes can...
10 October 2014 9,716 1 View
10 October 2014 7,765 8 View
I am working with Xanthomonas oryzae bacteriophages. When I try doing a plaque assay I can visualize the bacterial lawn after 2 days, but plaque formation is not seen. It takes almost 5 days for...
10 October 2014 3,339 11 View
We are going to test deer mouse fecal samples for glucocorticoid levels. However, we are unsure if grinding feces in methanol is adequate for viral inactivation. Any guidance/input would be great.
10 October 2014 6,822 2 View
Do they survive thawing? At what stages is it possible to freeze them and in what conditions? temps?
10 October 2014 8,861 8 View
Hi, I'm new in the field of virology and am looking into retrovirus (MLV) production at the moment. I've read vaguely that one can measure the titers by infecting NIH3T3 (gold standard) with...
10 October 2014 9,236 3 View
I've read some family viruses as Siphoviridae, Myoviridae, Podoviridae and Tectiviridae have members that are able to infect archaea and bacteria, however, members belong to different clades and...
09 September 2014 5,892 6 View
We are making a review on it and according to a review of Cheng in 2005 (CLINICAL MICROBIOLOGY REVIEWS, Apr. 2005, 18(2):383–416), melioidosis is only endemic in some areas of South East Asia and...
09 September 2014 4,114 12 View