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Questions related to Virology
ATCC used to have this product but it's been discontinued. I've searched everywhere and I couldn't find a company that provides FMV viral cultures etc.
12 August 2016 8,225 2 View
Read 7 answers by scientists to the question asked by Subhasmita Mohanty on Aug 10, 2016
10 August 2016 3,351 6 View
Hi,I have been setting a over-expressed Jurkat cell lines for months but I cannot get a stable cell line. First ,I transfected 4 plasmids into 293T cells and collected the supernatant after...
01 August 2016 4,728 6 View
Does Ebola leave a specific antigen upon infected cell membranes? If yes which antigen?
28 July 2016 8,283 2 View
Do we have to seal the lower part of the well with agar or just leave it unsealed
26 July 2016 5,846 4 View
Can anyone suggest me a alternative protocol for sporozoites purification method other than Accudenz column purification method? Or if you have the easy process for Accudenz column purification...
05 July 2016 1,657 2 View
I am confused about the calculation of TCID50/ml values for HIV virus. I am using 10,000 cells/well of TZMbL cells and 2 days of infection in in presence of 10microG/ml concentration of DEAE, as...
30 May 2016 3,852 3 View
What is differences between expression of haemagglutinin or neuraminidase surface antigenic in influenza viruses? Expressing recombinant HA have better antigenicity than neuraminidase?
27 May 2016 3,202 2 View
I expressed a hemaglutinin glycoprotein from Avian Influenza virus in an Adenovirus, I didnt see any HA, what would be the cause!?
23 May 2016 1,138 3 View
05 May 2016 7,038 2 View
Well, I want to express protein using OmpA on bacteria surface(outer membrane). so I extracted e.coli DNA from ETEC and then PCR to get OmpA. originally, I expected that the sequence of OmpA is...
05 May 2016 6,671 0 View
I want to prepare 1M CoCl2 to mimic hypoxia. But when I dissolved the Cocl2 (Simga, anhydrous) in the steriled water, it was difficult to fiter through 0.22um filter. After standing for minutes,...
05 May 2016 6,142 3 View
I am working to amplify a sample with coinfected similar RNA virus to check their competitive fitness. after viral rna extraction, then proceed to cdna synthesis. later i going to amplify both the...
05 May 2016 5,825 3 View
I'm currently attempting to individually clone the non-structural genes of a tick borne flavivirus, louping ill virus. I've had success with all of the NS genes apart from NS3 (1.8kb) and NS5...
05 May 2016 8,007 2 View
Effect on tumor growth, effect on T cell function.
05 May 2016 8,835 1 View
05 May 2016 5,235 3 View
05 May 2016 4,919 3 View
I'm preparing virus sample for transmission microscopy for the first time. What is the best way how to concentrate virus for transmission microscopy? From literature, I understand that the best...
15 April 2016 6,806 7 View
The author's describe their replicon and reporter as such:We screened for host proteins involved in HCV replication using the Huh7/Rep-Feo subgenomic genotype 1b HCV replicon that we had...
14 April 2016 7,342 2 View
04 April 2016 1,620 2 View
Hello, I wanted to know if there was a general test/s by which we could determine if there is a viral infection without having to specify which virus. Like with sequencing 16 s rRNA by NGS we can...
04 April 2016 3,581 6 View
04 April 2016 6,523 8 View
Thanks!
04 April 2016 7,123 2 View
It has been observed that certain laboratory models-- rabbits and few others developed spontaneous neurological symptoms upon inoculation with infectious Prion strains, could Murine Endogenous...
04 April 2016 3,951 2 View