09 September 2015 1 3K Report

I need to carry out the titration of the dengue virus by focus forming units (FFU). Does anyone know of someone article and which the formula to determine the viral titer?

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What other reagent could be used as a positive control of the Griess method?

I use LPS at 5ug/mL as a positive control in the Griess method, but nitrite production is very low.

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I can use fluconazole for eliminating fungi in cultured cells?

The fluconazole is effective in the prevention of fungi in cultured cells?

09 October 2016 5,872 1 View

Why do my VERO cells die after a while?

VERO cells die after a while. There isn't evidence of contamination (bacteria and fungi). In addition, other cell lines aren't killed and with the same culture medium VERO. Which can be?

02 March 2015 8,268 7 View

The SGR amplification curve in my qPCR does not appear. However, when I put the SGR qPCR product in agarose gel the amplicon appears. What happened?

SGR is a sample and PJFH1 is endogenous control. *The mixture was the same for both samples, changing only the primers.

02 March 2015 4,440 10 View

Has anyone of you managed to express plaque forming units (PFU) to dengue virus in Vero E6 or LLCMK2 cells?

I can not express plaque forming units (PFU) to dengue virus.

08 September 2014 9,531 4 View

Does anyone use the reading of 495nm to assess cellular viability in Huh7.5 cells (MTT method)?

MTT method

05 June 2014 9,921 5 View

Does anyone know the base sequences of human endogenous primers for real time PCR? Such as BAC1, HPRT and GAPDH.

Companies sell with probe, but I don't need the probe. How to get the base sequences of the endogenous primer?

01 February 2014 5,757 3 View

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