Discuss everything related to viruses | Contact experts in Virology to get answers
7,819 views 621 posts
Questions related to Virology
I have believed the specificity of viruses to their host species, based on the protein-protein interaction. But now am reading some papers suggesting that transmission across kingdoms is possible,...
04 April 2015 4,690 0 View
I want to make a large stock of my algal viruses and freeze aliquots to use throughout my experiment. Does anyone have a method for freezing viruses so that they can be kept for several months but...
04 April 2015 1,246 4 View
I'm looking for a tissue culture adapted Dengue-2 virus sequence that will propagate in tissue culture with no problems (for example in BHK-21 cells). I'm looking to make this virus using reverse...
04 April 2015 7,000 3 View
Briefly, my study will be on determining association of some qualitative risk factors among adults and paediatrics who are infected by dengue haemorrhagic fever in the same population. This is a...
04 April 2015 1,680 2 View
I am trying to amplify tomato spotted wilt virus by PCR from Chrysanthemum but sometimes the symptomatic plants doesn't amplify?
26 March 2015 2,102 7 View
Does anyone let me know about the methods to screen anti-HIV activity for new chemical compounds which are expected to act on HIV-1 reverse transcriptase. I require both in silico as well as in...
25 March 2015 8,452 3 View
This is a question for virologists, molecular biologists and biochemists. The import of HIV1 DNA has been shown to rely on the nuclear pore complex protein RANBP2. RANBP2 has several functional...
06 March 2015 6,226 2 View
I work with a viral non structural protein, an integral ER membrane protein whose structure is unknown. During viral replication the protein goes to the ER membrane and supposedly interact with...
05 March 2015 8,537 5 View
Guys please help me out. I was given the protocol below for inactivation of FLU A Pandemic 2009 H1N1 for the HA assay but I get no agglutination on the HA assay. For step 1. I take 4ml of...
04 March 2015 1,508 18 View
03 March 2015 3,472 6 View
03 March 2015 7,880 2 View
Guys I`m trying to set up the Influenza HI assay in our lab but I`m struggling to get any virus titre on the HA assay. I grew up H1N1 \California/07 pdm0 in MDCK cells. On day 3-4 good I saw good...
03 March 2015 6,883 13 View
03 March 2015 974 8 View
Pseudorabies virus is a pathogen of swine, it do not infect primate and human. However, the HSV-1, belongs to the same family with PRV, can infect human. Why does PRV do not infect human? Is it...
03 March 2015 3,534 3 View
The point of origin and insertion of mutation should be taken into consideration.
03 March 2015 6,257 3 View
Dear all, I was wondering for short-term storage of retroviral supernatant, should I freeze it at -20C or keep it at 4 degree? Thanks in advance for your kind suggestions! Yanran
03 March 2015 4,487 12 View
In recent years several viral diseases like bird flu,avain flu, swine flu ,ebola etc has called panic in several countries. Such diseases nolonger remain localized but, lilely to be spread...
19 February 2015 7,859 12 View
I have influenza virus produced in eggs and then purified by ultracentrifugation on a sucrose gradient after protein determination to know the amount of virus to put in the protein gel I run the...
19 February 2015 2,523 2 View
For ethical approval purposes, I need to address the following points. Is there a viremia in Vzv infected rats? If so How long does it last for? I have red quite a bit on the subject however I...
06 February 2015 4,884 3 View
Hi everyone, I meant to set up the postherpetic neuralgia model. I can`t find any evidence regarding the Vzv viremia in rats. Is the virus still functional? I am writing a biosafety application,...
06 February 2015 10,104 6 View
I am working on plasmacytoid dendritic cells and many references suggest that flu PR8 is best for stimulation of pDC...can anyone tell me if I plan to use this virus what kind of biosafety should...
02 February 2015 2,405 9 View
I googled for syncytial assay protocol. But I couldn't get proper hits for that. If anyone in this forum is aware of or routinely performing syncytial assay, I appreciate if you can share the...
02 February 2015 7,329 2 View
02 February 2015 9,707 2 View
02 February 2015 3,245 7 View