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Questions related to Virology
Prophage Induction with mitomycin C (1ug/ml) gave me reduction in bacterial turbidity measured at OD 600 nm. A spot test of the lysate had given a noticeable clearance. But there no plaque...
13 February 2017 5,136 4 View
we are trying to infect mice (BALB/c) with HSV-1F. but no visible changes are there on the skin like mentioned in literature (blisters on the area of infection). We administered intradermally and...
03 February 2017 493 4 View
I'm looking for an extremely sensitive method for detection/quantification of viruses. More sensitive than the traditional qPCR. Probably down to detection of 1 - 100 viral particles. Any thoughts...
01 February 2017 287 2 View
All papers only mention using supernatant of the cell culture. But wouldn't the FBS in the medium cause unnecessary binding?
27 January 2017 7,451 4 View
Why in HIV/AIDS CD4:CD8 ratio is considered? Why not CD8:CD4 ratio?
12 January 2017 2,326 3 View
hi everyone! what are the replication strategies used by viruses to replicate
11 January 2017 5,414 3 View
Hello, when a patient is first infected with Ebola my understanding is that there is not really a primary viremia and for the first week Ebola just sits in the organs and or white blood cells...
08 December 2016 4,700 7 View
The ICTV states on their homepage that taxonomy lies at the "uneasy interface between biology and logic". The latter is dependent on the former, not just in taxonomic classification, but in...
23 November 2016 9,991 3 View
The virus replication is evident at 6 to 8 hour if transfected with virions or viral RNA (Ann Rev Phytopath, 1975, 13:105-125). However, I would like to know the approximate time when transfected...
04 November 2016 6,780 3 View
Hi, I am having some trouble to do Focus formation assay in NIH3t3 cells. I start with making NIH3t3 cells stable using retrovirus (90% infection efficiency after 72hrs with pSIR-GFP virus). My...
31 October 2016 948 2 View
I typically hear UNC and UPenn. There must be something in Europe?
29 October 2016 5,482 3 View
Would anybody explain (with reference) why 1 OD=132 µg/ml incase of FMD virus?
27 October 2016 1,146 2 View
So, the problem is that I deleted 2 genes essential for plaque formation (I am talking about Poxvirus - Cowpox virus).I found information that Poxvirus can replicate only in the RK13 but without 2...
18 October 2016 3,582 3 View
Good day! I have a question. This is my first time that I am working with a primary antibodies. What can I use as a diluent for this? PBS?
09 October 2016 9,687 14 View
Part of my project is to compare the total number of influenza (B/Phuket/3073/2013) binding antibodies in human serum and mucus supernatant. Unfortunately, only mouse monoclonal antibodies for ...
27 September 2016 9,897 2 View
Is anyone actively researching Parechovirus? It is anticipated that the virus will again see some outbreaks this fall. Yet, I have not seen any US researchers or organizations publishing...
25 September 2016 8,167 2 View
hello, every one any one know what is the meaning of Tripartite leader sequence for mRNA in adenovirus transcripts or generally for gene transcription . thanks zeinab
16 September 2016 5,148 3 View
I'm looking for the strain of DENV-2 called 16681 (Dengue virus type 2 Thailand/16681/84), but cannot find it on ATCC. Most of the papers I find using it do not state where they obtained it from...
14 September 2016 5,242 3 View
Hi, I'm trying to produce pseudotyped lentiviral particles but I need high titers of lentiviral particles. I used ultracentrifugation for this purpose with a sucrose cushion, and the pellet it was...
14 September 2016 7,072 2 View
It is usual to find cases of herpes zoster as first time illness. is it possible that herpes zoster attacks the same patient after complete healing of first infection.
02 September 2016 9,989 11 View
What is the differences in reporting qRT-PCR result in term of delta delta CT value and absolute copies number? Which one is more appropriate to describe differences in cytokines level before and...
30 August 2016 7,582 4 View
I am looking for research references that could be applied into my research field. In your experience, which emerging viruses are likely containing a completely research system (the virus to be...
29 August 2016 6,549 1 View
Many scientists talked about first report of VNN in marine fish In many of scientific reports such as: 1) The first report of viral infection in Asian sea bass was made by Glazebrook et al....
26 August 2016 7,623 6 View
The trypsin can change the VERO receptors used by dengue virus infection.
18 August 2016 2,082 3 View