07 July 2015 7 10K Report

Hi! I'm starting to standardize the PCR, so I'm not really sure wich is the best DNA concentration or primers concentration that I need to use

Mzia 1(5' GATATTTGIGGIGTTCAGCCIATGA 3')

Mzia 2(5' CGCGGTTGATTTCCAGCATGATTTC 3')

According publish papers:

Initial denaturalization: 94  ºC x 1.5 min, 94ºC x 45sec

annealing: 45ºC, 50ºC, 55ºC, 60ºC x 1min

extension: 72ºC x 45 sec (30cycles)

final extension: 72 ºC x 1 min

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