Virology

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Questions related to Virology

Why are animal viruses better at developing resistance to countermeasures than plant viruses?

Viruses that cause human disease often become resistant to the drugs that we treat them with. In contrast, plant viruses seem to overcome countermeasures (such as resistance genes incorporated...

16 May 2018 221 3 View

What is the most recommended mosquito trap for mosquito surveillance purposes?

Hi everyone! My lab is in the process of establishing a new project involving the use of field-caught mosquitoes. Does anyone have any highly-recommended traps for catching mosquitoes in the...

06 February 2018 4,147 7 View

Which condition should be considered in Viral Nervous Necrosis (VNN) challenge?

One of my friends in Malaysia have tried 3 times challenged on their Grouper and Sea Bass fish with local NNV isolates, and all failed, meaning the fish did not die or show any clinical signs, but...

08 November 2017 9,179 2 View

Has anyone estimated the number and catalogued the extinct virus genera or species?

Viruses are obligate intracellular parasites of diverse hosts ranging from prokaryotes to eukaryotes belonging to all phyla. Several host species have gone extinct at different times, so must have...

27 July 2017 10,051 5 View

Recommended IgG concentration for preventing HSV-1 spreading through the medium?

In HSV-1 plaque assay, how much of pooled human immunoglobulin is enough to block secondary infection through the medium? I've found two HSV-1 plaque assay protocols with IgG, one recommending an...

24 July 2017 4,278 3 View

Has anyone worked with the Modified Vaccinia Ankara (MVA) virus?

I have a list of peptides from the Western Reserve strain of the Vaccinia virus. I wish to find sequences in the proteome of the MVA. I have tried BLAST, but it seems that the entire proteome of...

14 July 2017 9,898 3 View

Detecting homologous regions (hrs) in Lymantria dispar nucleo polyhedrovirus ( LdMNPV )

I would like to learn is there any software for detecting Lymantria dispar nucleopolyhedrovirus hrs regions? I am working on a full genome sequence data and I need to search hrs regions in my...

06 July 2017 3,831 2 View

Who is working with Tilapia Lake Virus (TiLV) and can send perhaps an isolate to my lab?

I have to be prepared if the virus comes up in Europe or Germany.

15 June 2017 5,687 2 View

Is it acceptable to do HI test using standard antigen of NDV after propagating it in the ECEs instead of buying new expensive one.

Usually we did HI test using NDV antigen (lentogenic strain) prepared by company. If we propagate this virus in ECEs it is approved to do HI test using the propagated strain instead of that...

13 June 2017 7,176 3 View

How to titrate PCV2 Virus (TCID50)?

Dear all, I'm currently working with PCV2 virus and want to optimize the titration protocol. Currently we don't have resources to do IFA or IPMA assay, so it will be based on CPE observation...

22 May 2017 2,376 5 View

Could polybrene can improve the efficiency of the infection of influenza A virus?

I want to use influenza A virus rescued from plasmids to infect MDCK cells. Could polybrene can improve the efficiency of the infection of influenza A virus?

12 May 2017 427 3 View

Newcastle disease virus contamination: how do we remove it?

Hello, please can someone guide me on what to do when the purified Newcastle disease virus got bacterial contamination?. Is there a way we can simply remove the contamination from the purified...

08 May 2017 8,626 6 View

I plaque a 24-well plaq. with C6/36 cells, but after 24h the cells didn't grow up. What can i do to work?

C6/36 cells, Arbovirus. Cell culture. Virology.

24 April 2017 7,913 3 View

What are other ways of increasing the effectivity of RT-PCR in testing for the Japanese Encephalitis Virus in mosquitoes?

I have read about RT-PCR as a molecular amplification assay for detecting the presence of flaviviruses. However, when used on the extracted RNA of mosquitoes carrying Japanese Encephalitis Virus,...

20 April 2017 3,164 3 View

What is the nature of the relationship between the three “parents” of the H1N1 virus?

The virus is said to contain genes from avian, swine, and human influenza A. How could a hybrid like this have happened? What events could have occurred to allow the reassortment of such genes?

18 April 2017 193 3 View

To rescure virus, will it be created a influenza A virus if i replace a protein expressed in H9N2 with the counterpart in H7N9?

To rescure virus, will it be created a influenza A virus if i replace a protein expressed in H9N2 with the counterpart in H7N9? For example, i transfect HA, NA, NS, M, NP, PA, PB1 from H9N2 and...

17 April 2017 3,519 3 View

Plaque assay for FMDV- Why are plaques not found?

I have performed plaque assay for FMDV. Agarose concentration was 0.8% and it was not low-melting agarose. The cell was perfectly monolayered. Under the microscope, plaque-like "big dot" in...

17 April 2017 1,626 3 View

How to determine the titer (TCID50) of chicken anemia virus in MSB1 cells

I am working with chicken anemia virus now, and I am using MSB1 cells. But it doesn't show clear and significant CPE, compare to negative control. So after I read some references, I did PCR for...

10 April 2017 5,289 4 View

Can someone suggest an optimum method for Retro/Lenti virus concentration?

Hi All, I have used Lenti-X™ Concentrator (Clonetech) to concentrate Lenti virus before, it worked but I'm not entirely satisfied. Can anyone suggest a more robust and an efficient way for...

03 April 2017 10,017 3 View

Sucrose gradient for Picornavirus purification?

Hi everyone, I'm working with Picornaviruses and I'm fairly new to purifying viruses. I've been trying to isolate full and empty capsids in a sucrose gradient for the past 3 weeks but no luck so...

30 March 2017 9,965 21 View

Virus Methods: Calculationg Virus titer and deciding which titer would be better for my experiment?

I was looking for this chapter titled 'Virus Methods' in a book 'Baculovirus expression vectors : A laboratory manual by David R. O'Reilly, Lois K. Miller, Verne A'. However, I do not have access...

28 March 2017 9,131 11 View

Capsid/Coat protein question?

My question is whether or not the sequence for a Phage capsid scaffolding protein (GPO) could be used use for the production of coat proteins. I know the scaffolding protein (gpo), is required for...

13 March 2017 9,372 1 View

White precipitation in virus inactivation?

Hi all, I have a problem regarding influenza virus inactivation with Formaldehyde. After incubation in cold room (4-8 ◦C), a white precipitate is formed. I haven’t got the problem before. Have you...

06 March 2017 9,385 7 View

One virus may have two different kinds of particle?

When we use Polyclonal antibody to detect a virus using western blotting， two kinds of virus particles were found ，while the virus we used have never been reported such case， I do not think this...

16 February 2017 765 10 View

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