What is your opinion about best moi of adenovirus for transfection of MCF-7 cell?

More Mohammad Shayestehpour's questions See All

Can anyone recommend a protocol for concentrae Adeno using PEG?

I need to a protocol for PEG-concentration of Adenovector or Adenovirus.

11 December 2015 9,180 1 View

Can you help me for Adenovirus CPE?

HI i transfected adenovector to hek293 cell line. i don't know Adenovirus CPE. this picture is hek 293 cell line 8 days post transfection. do you observe any CPE?

10 November 2015 5,225 4 View

Can you help me with miRNA mimic transfection and negative control?

HiI want to transfect 10 nM, 20 nM and 30 nM of miR145 mimic to MCF-7 cell line. Then I want to transfect a plasmid containing miR 145 target sequences at the end of my gene. I want to evaluate...

09 October 2015 2,323 6 View

Can you help me about problem with gateway technology and LR reaction?

I have a problem with gateway technology and LR reaction.I Perform LR reaction with various vector concentrations but the reaction is not carryout. I did check LR enzyme and this enzyme worked...

09 October 2015 8,039 2 View

Can you help me about adenovector for cloning and virotherapy?

I am working on virotherapy using adenovirus.I want to return E1A adenovirus (including promoter + CDS) to pad/pl/dest vector (invitrogen).If I return E1A gene (from nucleotide 400 to 1700 of wild...

07 August 2015 7,272 1 View

Is it possible that supercoiled plasmid migrate slower than linearized in agarose gel ?

I have been trying to clone a 1200 bp insert to PENTR vector.After transformation and plasmid extraction, I run the super coiled and linearized vectors on the gel.The length of linearized vector...

06 July 2015 617 3 View

Can you help me for solving the problem of plasmid-insertion deletion in Ecoli during cloning?

I inserted 3131 bp to a plasmid with 3600 length by ECORI and BSP119I enzymesAfter transformation of bacteria (TOP10) and replication, I purified the plasmid.Plasmid digestion and sequencing...

05 June 2015 1,132 4 View

Can anyone advise me on solving the cloning problem?

I inserted 3131 bp to a plasmid with 3600 length by ECORI and BSP119I enzymes After transformation of bacteria (TOP10) and replication, I purified the plasmid. Plasmid digestion and sequencing...

04 May 2015 4,058 2 View

Can anyone advise me on solving the cloning problem?

I inserted 3131 bp to a plasmid with 3600 length by ECORI and BSP119I enzymesAfter transformation of bacteria (TOP10) and replication, I purified the plasmid.Plasmid digestion and sequencing...

04 May 2015 9,774 6 View

Can anyone advise me on solving the cloning problem?

I inserted a 3131bp fragment (PCR product) to a vector backbone with 3600bp length by two enzymes ECORI and BSP. After plasmid extraction and linearization, the vector run on the Agarose gel. I...

03 April 2015 6,459 9 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

If we are using snowball sampling technique, how do we justify the true representativeness of the sample statistically? is there any statistical test?

Are there any statistical methods to justify your sampling technique using SPSS or AMOS?

05 August 2024 9,153 4 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

What are possible strategies can be used to analyze data under sequential explanatory mixed method approach?

Better ways to analyze the qualitative and quantitative data in a sequential explanatory mixed method approaches

04 August 2024 2,703 6 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Divaker Choubey

The optimum MOI depends upon culture conditions of cells. Therefore, an optimization may be helpful. One can use a range 1-10 virus/cell.

Mohsen Sarikhani

I have tried with MOI 5 and worked well..But that depends on the experiment and the level of expression you are looking for. however incubation after infection has to be considered.

may I know what do you wanna over express exactly?