Can anyone recommend a protocol for concentrae Adeno using PEG?

I need to a protocol for PEG-concentration of Adenovector or Adenovirus.

11 December 2015 9,107 1 View

Can you help me for Adenovirus CPE?

HI i transfected adenovector to hek293 cell line. i don't know Adenovirus CPE. this picture is hek 293 cell line 8 days post transfection. do you observe any CPE?

10 November 2015 5,150 4 View

Can you help me about problem with gateway technology and LR reaction?

09 October 2015 7,966 2 View

Can you help me with miRNA mimic transfection and negative control?

HiI want to transfect 10 nM, 20 nM and 30 nM of miR145 mimic to MCF-7 cell line. Then I want to transfect a plasmid containing miR 145 target sequences at the end of my gene. I want to evaluate...

09 October 2015 2,242 6 View

Can you help me about adenovector for cloning and virotherapy?

I am working on virotherapy using adenovirus.I want to return E1A adenovirus (including promoter + CDS) to pad/pl/dest vector (invitrogen).If I return E1A gene (from nucleotide 400 to 1700 of wild...

07 August 2015 7,118 1 View

Is it possible that supercoiled plasmid migrate slower than linearized in agarose gel ?

I have been trying to clone a 1200 bp insert to PENTR vector.After transformation and plasmid extraction, I run the super coiled and linearized vectors on the gel.The length of linearized vector...

06 July 2015 538 3 View

Can you help me for solving the problem of plasmid-insertion deletion in Ecoli during cloning?

I inserted 3131 bp to a plasmid with 3600 length by ECORI and BSP119I enzymesAfter transformation of bacteria (TOP10) and replication, I purified the plasmid.Plasmid digestion and sequencing...

05 June 2015 1,059 4 View

Can anyone advise me on solving the cloning problem?

I inserted 3131 bp to a plasmid with 3600 length by ECORI and BSP119I enzymes After transformation of bacteria (TOP10) and replication, I purified the plasmid. Plasmid digestion and sequencing...

04 May 2015 3,971 2 View

Can anyone advise me on solving the cloning problem?

I inserted 3131 bp to a plasmid with 3600 length by ECORI and BSP119I enzymesAfter transformation of bacteria (TOP10) and replication, I purified the plasmid.Plasmid digestion and sequencing...

04 May 2015 9,698 6 View

Can anyone advise me on solving the cloning problem?

I inserted a 3131bp fragment (PCR product) to a vector backbone with 3600bp length by two enzymes ECORI and BSP. After plasmid extraction and linearization, the vector run on the Agarose gel. I...

03 April 2015 6,379 9 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View