Unwanted Real-time PCR signal - probe degradation - if so why?

Hi All If you take a look at the image, the upper virus X assay (for Rotavirus) is giving off a lot of fluorescence at the end of the run (circled) which is clearly not real (we know these are...

02 March 2016 3,832 9 View

RBC choice for the HI assay?

can i use human type-o rbcs with h3n2 Texas and Victoria in the HI assay?

07 August 2015 7,666 3 View

Can someone help with the interpretation of Influenza haemagglutination inhibition assay results?

05 June 2015 452 9 View

Can someone help regarding RBC pelletet in the influenza haemagglutination inhibition assay?

Gi often get nice pellets forming but they sometimes don't seem to run when I tilt the plate, should I count them as antibody positive.for example streaming ofpellets has occurred up until the...

04 May 2015 7,183 3 View

Why does my dmem turn purple?

04 May 2015 9,174 10 View

Does anybody have a strain of MDCK cell line that they know will support replication of A/California/07/09pdm?

03 April 2015 2,526 4 View

Is there an optimum time point (day/hrs) to harvest influenza infected culture supernatant to obtain a high titre for the haemagluttination assay?

02 March 2015 6,488 13 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

How long do vaccines protect us against COVID 19?

Or will we have to get vaccinated every year, as in the case of influenza?

27 February 2021 1,048 5 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

How do you mesh a thin solid in Fluent?

Hello, I have used the shell function on SpaceClaim to create thin solids for the model I am studying. These are contained within an enclosure for my study. I chose this approach as it avoids the...

24 February 2021 8,856 3 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View

Do you consider undergoing a structure literature review / Meta analysis and getting numerical findings sort of quantitative method?

I am looking for a classification of the research methods included in literature review papers or Meta analysis ones .

22 February 2021 1,131 4 View

How to control the variants vof covid 19 in second and third waves?

A the world find means to control covid 19 by vaccination ,new variants came to spread and make the world in fear and danger, to understand the conduct of these variants, es constantly change...

21 February 2021 3,080 6 View