Has anyone used Iodixanol gradients to separate exosomes from virus?

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Syed A Ali

Hello Sharon,

Does the principal behind velocity gradient centrifugation change with type of material floated on such gradients?

David Fenard

dear Jessica,

you can find protocols with Optiprep online (apllication sheet V34) and some publications like this one:

"Discrimination between exosomes and HIV-1: purification of both vesicles from cell-free supernatants" (Cantin R et al (2008) J. Immunol. Methods)

best