If I would like to study the release of inactivated polio virus, how could separate the released polio virus from the carrier? It seem the size of the polio virus is too large for dialysis based method. BTW, do any one know the size and molecular weight of inactivated polio virus?
What is the physical stage of the inactivated poliovirus?The virus particle is fragmented or the viral particle remains intact but loss the ability of causing diseases?The size of the particle is about 30 nanometers; I will separate the fragmented viral particle as well as the intact particle by sucrose gradient ultra-centrifugation.The concern of intact inactivated polio virus is the safety issue, Are the so call “inactivated viral particles" still remain infectious?
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