Detection of hemoglobinopathies. Measurement of pathological hemoglobins HbA2 and HbF,can someone give me details about this topic?

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Does there exist a qualitative analysis software that scans the data to create themes or categories [relevant to content analysis]?

Much of the software I have examined only organizes data [interview data] but does not create themes or categories. The task of creating themes and categories is left to the human being...

31 January 2020 6,901 12 View

Getting CD 63 in extracellular vesicles at 100 kDA?

Hi, I am getting a very strong specific signal with abcam at 100 kDa for CD 63 extracted using exoquick. This was repeated multiple times with the same result. Most pubs declare size is between 26...

08 October 2019 3,028 1 View

How can I create an absorbing boundary condition for waves in soil with dashpots and springs?

How can I create an absorbing boundary condition for waves in soil with dashpots and springs? I have created springs and dashpots at the bottom of my soil model, but the analysis aborts because...

05 September 2018 9,012 8 View

Could someone tell me how efficient is the MiMIC technique for fusion proteins?

I would like to start using the MiMIC technique and I don't know how to deal with it yet. First, I'd like to know how efficient is this technique and how long it takes usually. Another thing that...

03 November 2015 8,871 0 View

Does someone know where to find a Zero Diffraction Plate for XRD sample: 50 x 50 x 2.5 mm , SiO2 single crystal, on side polished?

See above

29 September 2014 2,971 2 View

What are some reasons for the low rates of flu vaccination among older African Americans?

The literature indicates that older African Americans have the lowest rates of flu vaccination and I am conducting a qualitative study in an attempt to shed some light on the issue.

03 January 2014 5,836 5 View

Difficulty about creating "Conceptual Framework"

Overcoming the Difficulties Associated with Using Conceptual... I have been attempting to create a conceptual framework for my dissertation and the struggle is monumental. Here is what I have...

01 January 1970 8,814 6 View

How to measure accurate concentration of whole protein from RBC?

Although I tried to perform western blotting with protein extract from RBCs, I could not detect the interest of protein due to red background (maybe, heme or hemoglobin ) all over the lane. I...

15 May 2024 6,182 1 View

Hello, i need help about the Hemoglobin dissociation curve of fish effected by pH in water enviroment?

When the pH turn low (acidification) in salty-marine water so what change of the Hemoglobin dissociation curve? Is respiratory frequency of a marine fish increase when pH turn low than...

19 January 2024 5,311 1 View

How can i deoxygenate oxyhemoglobin solution using nitrogen gas?

I want to deoxygenate oxyhemoglobin with nitrogen bubbling, but i can't observe the characteristic peak of deoxyHb in UV-Vis spectrum. Also, the concentration of my hemoglobin sample was little...

01 January 2024 5,512 0 View

Troubleshooting direct sandwich ELISA?

I am validating an ELISA measuring A1c (also called glycated hemoglobin; target for humans) for use in another species. It is a direct sandwich assay. The standard and my sample (whole blood) were...

25 October 2023 3,471 4 View

I need a protocol to reduce any oxidized (Fe3+) hemoglobin in my sample to (Fe2+)?

Hi everyone, I’m using hemoglobin in the lab and I need it to be in the reduced state (so it is able to bind with CO2). The hemoglobin I have is a lyophilized powder from Sigma Aldrich (H7379)....

13 September 2023 9,133 4 View

Has any previous research been conducted to evaluate the impact of hemolysis, jaundice, and hyperlipidemia on IR spectral profile in serum and plasma?

Only when hemolytic anemia results in a two- to threefold or larger increase in hemoglobin catabolism does jaundice owing to rise of serum unconjugated bilirubin become noticeable. The level of...

07 September 2023 3,103 0 View

I made a lytic reagent for 3-part cell counter. How can I reduce the hemoglobin percentage?

All parameter is ok but hemoglobin is 4% higher than control. the formula is SDS + Triton x100+ phosphate buffer.

11 July 2023 8,274 2 View

MRNA de novo assembly: How good is Geneious de novo assembly of transcriptome data using their assembly function (or other suggestions for assembly)?

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10 July 2023 3,841 3 View

How to deal with different adn lenghts in the making of a phylogenetic tree?

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18 June 2023 7,881 2 View

Does hemolysis interfere with spectrophotometric analysis by Methylene blue?

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19 April 2023 5,769 2 View

Fahad Alshehri

It is automated procedure in most of hematology lab called Hb Electrophoresis using HPLC. Simply you can search in google and you will find a huge literature about this technique. However, if you have a specific question or interpretation of rare disease that is will be worth.

Felipe Chagas

search for hemoglobin electroforesis differences when performed under acid or alkaline gel, different techniques, how to relate this to diagnosis or medications in use, different methods for hb electroforesis, relation with transfusion and drugs, molecular weight and its interaction with the eletric charge of the hb molecule under study, when to approach with different genetic tests and their relevance/accessibility/real life impact/cost