I wonder if the wizards of qPCR stats can help me with this one.

It is quite common the in the field of human genetics to identify a patient with a particular mutation and do qPCR to assess whether gene expression is altered.

In these cases, a single patient is compared to >=3 healthy controls.

What kind of statistics would be the most correct in this scenario to , for instance, show that a gene is less expressed in the patient than in healthy controls?

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