Is puromycin used in transduction with human peripheral blood mononuclear cells (PBMCs)?

15 December 2024 1 10K Report

Puromycin would be used to select for transduced cells after the fact. it is a mammalian selection agent so you plasmid/virus would need to express with Puromycin resistance gene then the other cells would die off. do a kill curve with untransduced cells to determine the dose/timing to effectively kill the cells then apply that 1-2 days after transduction

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Science method

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