To analyze sodium benzoate powder using UV-visible spectrophotometry, dissolve it in a suitable solvent like water or ethanol, preparing standard solutions for calibration. Measure absorbance at around 225 nm to obtain the spectrum and quantify the concentration.

Khondaker Touhidul Islam

Kindly check its chemical properties from literature and further solubility. Refer book analytical Profile of Drug substance if available where you will get all drug profiling right from UV-Visible scan ,XRD Graph, NMR,HPLC etc.

Prepare a "X" ppm concentrations solution of Sodium benzoate and scan it to over the range of 200nm to 400nm so that you will get spectra and lambda max. Check absorbance should be 0.6 to 1.2 is ideal. Beyond can go low and higher provided beers' lambert law should follow.

UV-Vis Spectrophotometric Analysis of Sodium Benzoate

Sodium benzoate, highly soluble in deionized water, exhibits λmax at 225–230 nm. Prepare a 100 µg/mL stock solution by dissolving 100 mg in 100 mL water. Dilute for calibration (10–50 µg/mL). Use quartz cuvettes (1 cm path length) and scan 200–400 nm. Measure absorbance at λmax, plotting concentration vs. absorbance. Ensure linearity (R² ≥ 0.999). Avoid organic solvents like ethanol. Maintain consistent conditions, using water blank. Deviations indicate impurities or matrix effects. Apply Beer-Lambert’s Law for quantification. Calibrate the instrument and validate results before interpretation.

To analyze sodium benzoate powder using UV-visible spectrophotometry, dissolve it in ultrapure water as the solvent. Measure the absorbance at around 225 nm, where sodium benzoate shows significant absorption.

The Best methods are by HPLC with C18 Column 25 cm by AOAC method its good to separated Na-benzoate and K-Sorbate but in manual food additives you can use the UV-Vis spectrophotometer