I would like to measure the area and diameter of thyroid follicles on a photomicrograph using image J (see attached image). Does anybody have a simple method for doing this given the large number of follicles in a single image?
What I am specifically hoping is possible.
1) Get an area/diameter for the entire follicle including the epithelium lining (in the high mag image this is the dotted black line)
2) Get an area/diameter of the colloid (blue dashed line and star region)
I would like to do this for the low magnification image attached that shows the large number of follicles separated by "interstitium" which is connective tissue and blood vessels.
It can absolutely be done with ImageJ or FIJI or other softwares. Lots of people do it by drawing each cell which is very time consuming and needs to be done accurately. FIJI and ImageJ have plug-ins available online and one might be a good fit for you to semi-automate this process. I personally use a plugin that detects CSA automatically based on membrane staining in muscle. Check out their library to see if one could help you save time and increase reproducibility.
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- Biological Science
- Histology
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