How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Can A. thaliana splice algae genes?

24 January 2021 329 2 View

Why my deltaATG mRNA is in polysome fractions ?

Hello everyone, I'm doing polysome profiling to answer a specific question. I study a gene which produce an alternative transcript and it's translation is controversial (extremely lucky if you...

16 December 2020 7,622 2 View

Agarose gel electrophoresis: only ladder is showing up?

07 December 2020 4,808 3 View

I am facing consistency issue in Hac-1 Splicing?

I am studying Unfolded protein response in yeast and I am checking Hac-1 mRNA splicing through semi RT-PCR followed by PCR. But I did not found any similar pattern of Hac-1 splicing in at least...

03 September 2020 7,346 2 View

Does splicing of a plasmid increase transfection performance?

Hey, I heard several times in the lab that a plasmid performs better (higher transfection efficiency, more protein produced, longer present in cells, ...) when the plasmid is spliced. (Meaning...

06 May 2020 7,454 3 View

How to splice fibres with different core sizes, e.g SMF(8.2um) to GIMF(62.5um) with minimum loss?

how to spliced different different core size (MFD) fibres ( single mode to graded index multimode). I am trying to splice SMF to GIMF, to fabricate SMF-GIMF-SMF saturable absorber. Although, I...

31 January 2020 4,223 6 View

Where can I find processed dataset of "Arrhythmia", "Ecoli" and "Splice"?

I'm an undergraduate student and my research topic regarding handling an imbalanced dataset. For the implementation, I need few datasets and I already have few. But I need a few more processed...

31 December 2019 5,795 3 View

Where can I find processed dataset of "Arrhythmia", "Ecoli" and "Splice"?

I'm an undergraduate student and my research topic regarding handling an imbalanced dataset. For the implementation, I need few datasets and I already have few. But I need a few more processed...

31 December 2019 2,451 3 View

What is the best method for mapping mixed prokaryotic-eukaryotic multi-species transcriptomes to reference genomes?

I am studying how a marine sponge holobiont reacts to particular environmental conditions (Differential gene expression in a treatment - control study). To do so, I sequenced 12 samples that...

11 December 2019 5,071 2 View

How to delete plasmid genes from Listeria monocytogenes?

Hello, I am aware that genetic engineering in Listeria monocytogenes is limited and am unsure of how to proceed. I would like to delete a 250 bp gene from a large (90 kb) low copy plasmid. Does...

10 November 2019 3,795 1 View