05 September 2023 1 4K Report

I am trying to prepare samples for ATAC sequencing and the requirements. of the company is 15 nM in 20 ul volume.

I ran the qubit to measure the concentration of the tagmented DNA and it was for example 7.72 ng/ul

second I ran the samples with bioanalyzer and I got average fragment size(bp) (416 bp)

I calculated the nM with this equation nM = (ng/µL x 106) / (660 g/mol x average fragment size bp).

I got 28.11 nM

My QUESTION: is it molarity per ul ? How to fix the samples to meet the requirements of sequencing company?

Thanks in advance,

Hager

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