I am trying to prepare samples for ATAC sequencing and the requirements. of the company is 15 nM in 20 ul volume.
I ran the qubit to measure the concentration of the tagmented DNA and it was for example 7.72 ng/ul
second I ran the samples with bioanalyzer and I got average fragment size(bp) (416 bp)
I calculated the nM with this equation nM = (ng/µL x 106) / (660 g/mol x average fragment size bp).
I got 28.11 nM
My QUESTION: is it molarity per ul ? How to fix the samples to meet the requirements of sequencing company?
Thanks in advance,
Hager