Our prodrug is activated through ROS-mediated reactions, particularly with H2O2, as indicated by our in vitro data. I'm currently working on designing an experiment to effectively reduce intracellular H2O2 levels, enabling us to establish a correlation between prodrug activity and the concentration of H2O2 within the cells. Up to this point, we've tested NAC, GSH, and impermeable catalase, all of which have proven ineffective. Catalase did show some capacity to quench intracellular H2O2 but was highly effective in extracellular spaces.

Any suggestions or insights on this matter would be greatly appreciated.