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Questions related to Staining
We want to do cd68 staining on human cell culture grow on coverslip. However, we are still waiting for the ordered antibody to arrive. So my question is, what is the best way to fix these cells...
02 April 2021 3,582 4 View
I'm trying to test the viability or dormancy of fungi spores, specifically Pisolithus species. Which are the best staining techniques for that? Thanks in advance
31 March 2021 9,582 10 View
Hi everyone, I have a DNA-FISH probe of 3 kb in length. I'm used to use very long probes (>100 kb) and hybridize them overnight at 37 C. Now I have to perform a FISH protocol using this 3 kb...
31 March 2021 3,838 1 View
I am trying to visualize the cranium vault morphology of 1mo old zebrafish stained with alizarin red. I tried just to dissect the cranial vault with a razor blade, however, I crushed the entire...
30 March 2021 5,932 2 View
Good evening, I need to prepare a staining solution for lipid bodies in roots following the protocol given by Brundrett et al. (1991). Here is what the article says: "(1) A sufficient amount of...
28 March 2021 3,496 2 View
For elucidating the mitochondria-specific superoxide of sperm MItoSox is to be used. how much dose of the same has to be used and what are the ideal results that are expected and what should if...
27 March 2021 4,956 3 View
Good morning, I recently got a problem with IHC slides on Ventana BenchMark GX. The sections (breast biopsy) changed dramatically but only for some of the slides, not all. I will give an example...
23 March 2021 5,714 5 View
I am seeking advice on optimization of pERK IC staining. I've been trying some experiments where I am transfecting different GFP plasmids into HEK293 cells that should substantially increase pERK...
23 March 2021 5,565 1 View
Hello, I am currently trying to stain for EdU in 40 micron thick sections brain sections. We have the EdU-Click it Kit but the protocol from the vendor only talks about cell stains not whole...
22 March 2021 5,494 3 View
I recently tried an ERa immunofluorescent stain using strep488 as the secondary. There are clearly ERa positive cells, but I'm not sure what these "dots"/noise are in the background of my GFP channel.
22 March 2021 7,039 10 View
Hi, I am currently working on a project that requires identifying the two subpopulations in mice using flow cytometry and I am having difficulties in my gating strategy. I am staining with CD45,...
18 March 2021 8,686 2 View
Hi! I want to make a direct co culture experiment with mesenchymal stem cells from dental pulp and bone marrow. To distinguish between them, I want to use some fluorescent tracer (bought...
17 March 2021 7,564 3 View
My research has shown that PD-L1 expression is significantly higher on non-classical (NC) monocytes in patients compared to controls, which was not seen on intermediate (IM) and classical (CL)...
16 March 2021 4,268 2 View
Dear collegues, how do you would perform Immunofluorescence staining on thick brain slices (around 400 micrometres) upon acute slices pharmacological manipulation or electrophysiology? -cut and...
11 March 2021 1,627 2 View
I've been staining 6um sections of FFPE human spinal cord tissue with fluorophore conjugated primary antibodies for multiplex immunofluorescence experiments and have noticed extensive non-specific...
11 March 2021 294 5 View
I want to do cartilage staining on Xenopus embryos (st 45). Following the protocol, I tried to dissolve 4 mg alcian blue in 7 ml ethanol plus 3 ml acetic acid. I found alcian blue powder is very...
10 March 2021 4,280 2 View
I am extracting hemolymph from bees and using Giemsa stain so that I can differentiate and count hemocytes. Do I need to apply a mounting medium and coverslip to my slides after staining to...
08 March 2021 462 3 View
I have approximately 400 slides that I need to stain. A colleague in the same lab has another 150 which need the same staining. Is there a way to stain 40-60 a day or are we just looking at a...
08 March 2021 3,089 2 View
I am currently trying to screen for coculture biofilm-formation of Bacillus subtilis pellicles (air/liquid-interface or "floating" biofilm). For this, I am trying to visualize the EPS of Bsub...
04 March 2021 9,021 3 View
We want to do multiplex immuno staining. Can anyone comment on pro and cons for CycIF vs iterative indirect immunofluorescence imaging (4i)?
03 March 2021 8,673 2 View
We used trypan blue 0.5% staining but did not seen a significant difference between live and dead Toxoplasma gondii under an optical microscope. In the articles, the use of trypan blue 1% and...
02 March 2021 8,994 1 View
Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...
28 February 2021 4,127 2 View
Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...
27 February 2021 9,369 3 View
I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...
26 February 2021 8,388 3 View