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Questions related to Staining
I found that lemongrass has a carbohydrate, but i can't find what type of sugar in it.
25 May 2023 9,149 2 View
in antimitotic activity, cells of root tip get stained during geimsa staining but nucleus does not get stained, why?
24 May 2023 4,903 0 View
I am trying to make rubber films from Natural rubber. Unfortunately, I don't have access to extruders or similar equipment and therefore work with solvents. As for my curing system, I am using...
23 May 2023 6,389 5 View
I'm doing a flow experiment, flowing single MCF7 cells through PDMS microfluidic chips (connected to a glass microscope slide). Currently, I'm using a Pluronic F127 (0.1 wt%) coating. However,...
22 May 2023 9,798 3 View
I want to isolate single cell clones of HepG2 from the pool using limiting dilution cloning (LDC) in 96-well plates. But I found that it's hard to get single cell suspension of HepG2 (at least 30%...
22 May 2023 633 5 View
Hi All, Recently, I've used BIOP JACoP plugin to analyse the protein protein colocalization during mitosis (mitotic cells, treated with Eg5 inhibitor). I struggle to understand the output of the...
22 May 2023 3,410 0 View
I want to the possibility of integrating the cosmovision underpinnings in ethnomedicine.
20 May 2023 8,364 0 View
Greetings, community! I am mastering visceral pain experiments. We are looking for an easy way to detect visceral pain specific behavior in mice. I have found a couple of articles in which...
19 May 2023 8,603 2 View
Hi. I need help to ID this white colony (4-7 days old at room temp). If more than 7 days of incubation, it turns green on top of the PDA agar and yellow at the bottom.
19 May 2023 1,619 1 View
Hi, I am trying to use MATLAB gamultiobj, but I need the variables to be between -1.5 and 1.5, but after it runs I realize it didn't take them into account. The variables exeed the conditions. I...
18 May 2023 7,945 2 View
Dear peers, I have a technical question about monitoring tumor bone metastasis. We want to establish a tumor model of bone metastasis and monitor the metastatic procedure. I am aware that IVIS...
17 May 2023 1,825 0 View
I'm doing an IF experiment to show the GBP (guanylate binding protein) protein level (cytosolic protein). However I'm having trouble doing my experiment, the fluorescence signals are expressed in...
15 May 2023 2,378 4 View
Hello everyone, lately I'm working with spheroids of primary mice intestinal stem cells. I'm trying to set up some 2D culture as well, trypsinizing the spheroids and seeding the individualized...
15 May 2023 8,415 3 View
antimicrobial test
14 May 2023 1,612 0 View
Unexplored traditional knowledge systems and techniques in Indian handicrafts and art forms encompass a wide range of practices. These include: Textile Weaving: Unexplored techniques in textile...
12 May 2023 934 0 View
hematoxyline and eosin are routin stain
06 May 2023 371 2 View
Hi all, I'm trying to evaluate the amount of viable E. coli cells in a colture. I'm going to use the Nuclear Green LCS1 and I will use a plate reader to evaluate the fluorescence emitted by the...
05 May 2023 6,052 2 View
Hi My experiment is to look at the intracellular location of a small peptide. I have difficulties when I try to do the IF staining for the small peptide that has been uptaken by cells, and the...
04 May 2023 8,891 2 View
there are many types of carbohydrates(glycoprotein ) :neutral and acidic glycoprotein
01 May 2023 7,600 2 View
My research direction is germination of actinomycete spores,and I can't tell if the spores have germinated under a light microscope at 100X.
30 April 2023 5,650 2 View
While collecting primary data with the help of a structured questionnaire, which is the best way to collect information on income/saving/expenditure? For ex: 1. Amount spent on the purchase of...
27 April 2023 7,481 2 View
I am preparing to do intracerebroventricular injection in mice, what is the volume of cerebrospinal fluid in mice?
26 April 2023 8,754 1 View
Hello, We are currently measuring concentrations of protein kinase A (PKA) and its phosphorylated from (p-PKA) in sub regions of the hippocampus using α-tubulin as loading control. We are using...
25 April 2023 6,587 0 View
Could anyone please provide me with the information on digital pathology slide scanner? Having computer programming software.
24 April 2023 9,141 1 View