If we want to culture blood cells of some wild species for karyotyping. Does we culture it after 48 hours or if not then what are the other possibilities for doing this.
If it is a case of small samples and little dna then whole genome amplification kits are very good for generating large amounts of dna of pcr-able quality although they are not cheap
Hello Sheikh Ahmed Gull
No, you can’t.
The maximum time is up to 24 hours in the preanalytical phase for karyotype test after which it may endanger the quality of results.
For more information, I would like to draw your attention to the article attached below.
https://www.scielo.br/j/jbpml/a/Nb3KX3Sh9xJCWngFDhCfD4Q/?format=pdf
As per the paper, three cultures were grown from each subject. The first, just after collection (0 hour); the second, 24 hours after collection and the third, 48 hours after collection. During the interval between initiation of a culture and another, the sample was stored at 4°C. The investigators concluded that the pre-analytical phase and the culture time of venous peripheral blood after collection are fundamental for the quality of the karyotype test. It was observed that there is a safety margin of up to 24 hours after blood collection, and after this time, cellular viability decreases significantly.
Best.
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