Irregular Baselines & Negative Fluorescence Units (FU): Some graphs show negative FU values, which suggests instrument calibration issues or improper sample loading.

Unexpected Peaks & Smearing: Some lanes (e.g., Lisa vdjb1, vdjb2, vdjb3) show sharp peaks that could indicate contamination, primer dimers, or salt carryover. Smearing in cDNA samples suggests RNA degradation or improper reverse transcription.

Broad Peaks in Some Samples: The "bibek" samples have broad peaks, which could result from high molecular weight DNA, overloaded samples, or poor sample quality.

Multiple Sharp Peaks in Some Lanes: This could be due to non-specific amplification, adapter dimers, or incomplete clean-up after PCR.

Thankyou