What is the best kit for DNA genome extraction from bile sample?

Kate Janicka Macasaet @Kate-Janicka-Macasaet

30 October 2024 1 8K Report

What is the most suitable DNA extraction kit for isolating bacterial DNA from bile samples: the widely-used QIAamp DNA Stool Mini Kit from Qiagen or the more affordable Vivantis GF-1 Bacterial DNA Kit?

Shahria Asrafi Snikdha

We used extractionsolution i, ii and iii which we made by using several chemicals in our lab.

Badges
Science method

More Kate Janicka Macasaet's questions See All

For anyone that works with Human Aortic Smooth Muscle Cells(HASMCs), what seeding density do you use when plating?

I am struggling to find a seeding density that will allow my cells to not aggregate together. Anything helps!

24 July 2024 3,460 1 View

Procedure of Ammonium sulfate precipitation of ACE2 in Calu-3 cells?

Hello, I am trying to find a protocol that looks at the extraction and purification of ACE2 inside the cell. We have the protocol for the affinity chromatography, but we are having trouble...

23 May 2024 4,342 1 View

Itc experiments procedure?

I'm a beginner in ITC, does anyone have an idea why when measuring the heat of dilution and in measuring the interaction of HSA with the drug or even with the buffer itself, the curve goes up? HSA...

28 April 2024 2,113 3 View

Can anyone identify these Cumaceans?

Hi everyone, I've been struggling to identify the family these guys belong to. Does anyone have any ideas? Taken from NE Atlantic 2200m depth.

23 April 2024 1,282 4 View

When washing demineralized fish scales, should I be concerned when there are string like substances (most probably fibers) are present?

My study is about synthesizing fish scales into chitosan and I am currently washing my demineralized fish scales. My sample fish scales were size reduced beforehand and when 1% HCl was added, it...

16 April 2024 2,129 0 View

In assessing the viability of using lithium heparin from gel separatory serum, what other tests can be added as parameters to further the study?

In the present day, countless tests are being run in the clinical laboratory. In the Journal of Oguzhan Zengi entitled, "The Transition from Gel Separatory Tubes to Lithium Heparin Gel Tubes in...

10 April 2024 3,541 1 View

How to make a thesis introduction?

how to start my thesis introduction

24 March 2024 2,366 1 View

What are some economical methods which can be employed by tertiary hospitals to develop & utilize regional antibiograms in the Philippines?

In the Philippines, national databases on antibiotic resistance are noted through the Department of Health (DOH) Antimicrobial Resistance Surveillance Program (ARSP). However, community or...

29 February 2024 4,600 1 View

What's wrong with Oil red O staining of the liver slices?

Hi everyone, the attached picture is oil red o staining of mouse liver slices after GAN diet, which was to induce NASH. I am wondering what are those round empty space with the clear boundary? Are...

27 February 2024 5,743 0 View

Fixed brain in 30% sucrose solution froze at 4°C?

I've been trying to figure out what happened but it seems I am the only one this has happened to? Processing: - transcardial perfusion with 1x PBS followed by 4% PFA - post-fix brain in 4% PFA...

12 February 2024 5,963 2 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

The question is how to use Wavenet transform?

HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?

03 August 2024 7,829 0 View