Is CARBOSEP CHO782 column(HPLC) is compatible for using 5mmol sulphuric acid as mobile phase instead of water? Or any method is available to analyse acidic samples in same?
4% H2SO4 based sample is neutralized using calcium carbonate and subjected for HPLC sugar analysis. But the neutralized sample contains trace amount calcium carbonate with it. Hence, a strong drift disturbs the baseline during the elution of the sugars(Retention time -12 to 14 in the picture) which makes difficult to determine the sugar concentration.
Is there any method to reduce/remove the calcium carbonate to remove the drift? Or else whether the column is compatible to use 5mmol sulphuric acid as mobile phase? Or any methods/ protrocols / suggestions to overcome this issue?
First, confirm CARBOSEP CHO782 column compatibility with 5 mmol sulfuric acid with the manufacturer. If compatible and needed, switch to NaOH/KOH neutralization, improve filtration, or use HPLC software for baseline correction to minimize calcium carbonate interference.
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