Is there any standard criterion to select optimum number of sequence candidates for establishing a phylogeny tree in bacteria using MEGA?

More Aniket Limaye's questions See All

Is there a way to map gene expression data from real-time PCR on gene trees?

I am trying to understand how the expression of paralogues changed over time. I have real-time PCR data and gene tree. I want to reconstruct the estimated gene expression level at each node. I...

14 March 2024 8,131 0 View

Why is my cloning not working?

I am using a pET28B vector to clone my 2.1kb insert digested with BamH1 (fwd) and Xho1 (rev) restriction enzymes. I'm using over 1ug of DNA for restriction digestion, kept at 37°C for over 3...

09 January 2024 984 4 View

What is the mechanism of halophilic microbes in remediation of salt affected soil?

Halophilic microbes, also known as halophiles, are microorganisms that thrive in high-salt environments. They have unique mechanisms that enable them to play a significant role in remediating...

20 September 2023 6,026 5 View

Problem facing while doing a Fatigue Analysis?

Hello everyone, I'm not able to do fatigue analysis of my model in ANSYS workbench, because I need some values .The material that I selected is ABS High Impact. I attach a file please check and...

19 September 2023 6,539 0 View

How to calculate energy consumption by c++ code?

What are the methods and algorithms available to accurately calculate the energy consumption of a C++ code? Please provide a comprehensive explanation including the relevant factors, metrics, and...

24 June 2023 5,564 0 View

Has anyone used Comprehensive Meta analysis software?

I am not able to construct the funnel plot. As well as should we use even values or event rate value?

07 May 2023 6,219 1 View

How to grow Campylobacter jejuni in lab?

I have tried them growing on Bolton Broth and then growing them on Campy Cefex Agar, and Blood Agar, under the microaerophilic conditions using anaerobic bag. But no growth.

01 November 2022 8,206 1 View

How to apply cyclic loading to beam column joint in ansys for a give experimental work and produce hysteresis curves?

Joint is strengthened using jacketting and gravity load is applied to it. Cyclic load is applied like given below : The test was carried out in two phases: in the first one, the gravity load on...

04 July 2022 5,848 0 View

How do I convert a Conc. in µg/ml to µg/g of wet weight?

I wish to estimate total protein content and further utilize the same to calculate total antioxidant activity.

15 June 2022 308 4 View

Primer of qPCR efficiencies without deltaRn values (DART, LnRegPCR)?

Hi Everyone, I am doing quantitative PCRs to estimate the expression of protein coding genes. I have two questions about estimating primer/PCR efficiencies directly from amplification...

08 May 2022 964 1 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

P. V. Arya

There is no harm in running with majority of similar sequences. It may be possible to design the similarity or dissimilarity criteria during the final phases of analysis

Aniket Limaye

Thank you very much. Is there any literature explaining about node score and distantness of neighboring bacteria?