The goal is to a align a high number (100-200) of microbial genomes(1-2Mbp) to analyze conserved regions.
Online servers will not be able to analyze to this huge data. It can be done by stand alone version of multiple sequence alignment tool.
Dear
Please check some infromations concerning sequence alignment:
-Jaroszewski, L. et al. 2011. Nucleic Acids Res. 39(Web Server issue):W38-44).
-Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ; Gish; Miller; Myers; Lipman (October 1990). "Basic local alignment search tool". Journal of Molecular Biology. 215 (3): 403–10.
-Hauswedell H, Singer J, Reinert K (2014-09-01). "Lambda: the local aligner for massive biological data". Bioinformatics. 30 (17): 349–355.
Thank
http://molbiol-tools.ca/Alignments.htm
Hello
I have never heard of such online services. How about stand-alone software?
Mauve (http://darlinglab.org/mauve/mauve.html) is a classical tool made for multiple genome alignment. If you need faster software, you might want to try Mugsy (http://mugsy.sf.net/).
Good luck
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