How to calculate concentration in nM from ng/ul qubit concentration and size base pairs after bioanalyser results in ChiP sequencing?

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Jochen Wilhelm

You can assume an average molar mass of 660 g per bp.

Manjul Rana

Thanks

Alaa Youssef Farag

@jochen did you mean that we divide the conc (ng/ul) per 660 ? Or should we consider also the no. of base pairs of the plasmid?

I am sorry but I am very confused.

If my linearized plasmid (7350bp) concentration is 100ng/ul, so the equation is like the following;

(100÷ (660* 7350) )* 10^6 = conc.(nM) ?

Is that right?

You don't just divide by 660. You divide by 660 g/mol bp. Mind the unit. If you take care about the units, it will be clear what's right and what's wrong. This should help you to resolve your confusion.