Any information about long chain PCR?

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Katie A Burnette

What organism are you using? I've attached a paper with good information in the supplement about using long PCR to detect deletions in nematode mtDNA.

The short answer is: buy a good quality enzyme, do a LOT of trouble-shooting, resign yourself to overnight PCR reactions, and keep telling yourself you can't rush quality results. Good luck

Article High Rate of Large Deletions in Caenorhabditis briggsae Mito...