Absorption coefficient of atmosphere at sea level versus wavelength?

I'm looking for some raw data for the transmission through the air in my lab, specifically in the MIR region. I've found a lot of data online that gives transmission vs wavelength, but the units...

22 December 2020 9,039 2 View

How to test if two subsets of a distribution have different correlations/regressions with other variables?

I have numerous non-normal distributions that, for the most part, have the same shape (n = 190). The variables are test scores and have a floor value of 5 and maximum of 50. They appear to be...

22 October 2020 9,305 3 View

Plant checklists for Borneo and Sumatra?

Dear RG Community, Can someone please give me references to publications (or online websites) that include plant checklists (vouchered if possible) for Borneo and Sumatra? Thanks in advance, Zach

20 September 2020 6,202 4 View

Should I use square transformed mulitple regression or ANOVA?

I am building a model with: - Non-normal continuous dependent variable (a score on a questionniare which does not have literal clinical meaning) - it appears fairly normal on histogram but there...

13 August 2020 3,439 9 View

Adding a transcriptional repressor before a promoter sequence in my plasmid?

I am performing a KI experiment using CRIPR/Cas. Our 5' homology arm is the 5'UTR of the gene of interest and therefore contains the endogenous promoter. I am looking for a repressor sequence to...

19 May 2020 5,088 3 View

How to design a vector that uses loxP for removal of blasticidin resistance gene?

Using CRISPR/Cas9 and homoly directed repair I have knocked in a degron tag onto the start of a protein of interest in 293T cells. WB has confirmed that ~5% of protein alleles have recieved the...

17 March 2020 4,470 1 View

A heterogeneous fluorescence sensor appears to exhibit second order scattering. The "scattering" responds linearly to target analyte, is this normal?

I am performing experiments on a suspension of fluorescent material that is quenched in the presence of a target analyte. I excite at 279nm and see a peak around 560nm. When I add analyte solution...

10 February 2020 2,743 4 View

What are the techniques to validate qualitative research instruments?

I am currently doing a quasi-experimental research. I have decided to use both qualitative and quantitive methods in collecting the data. For the qualitative data, I have an observation card and...

08 October 2019 4,007 5 View

How a researcher can validate his upcoming research instruments?

I will be conducting an action research in one of the schools at my place of working and my supervisor has asked me to validate my instruments before I can use them during the research process. I...

11 December 2018 1,355 3 View

Bizzare qRT-PCR amplification curve - Why does signal drop to zero near end of run?

Sorry for the poor image quality, I am working with a fairly old real-time PCR system (Bio-Rad MyIQ on iQ 5 version 2.0 software). I am using primers, cDNA, and supermix (Bio-Rad iQ SYBR Green)...

12 August 2018 3,553 4 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

No title

02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View