I need a frequently curated database of Bacterial Insertion sequences which can be downloaded and used as input for standalone local BLAST to ascertain the presence of Insertion sequences in the Genome of Interest.
Wouldn't really know if it is a comprehensive one, but the IScan tool available at http://www.bioc.uzh.ch/wagner/publications-software.html does identify transposase sequences, inverted repeats and candidate target direct repeats of ISs in complete genomes.
This paper might help you in figuring it out : A survey of bacterial insertion sequences using IScan, Wagner et al.