There are some >150 types of HPV (human papilloma viruses); I am looking for the conserved regions from their genome.
you can retrieve the sequence of all the available isolates and do a multiple sequence alignment using MEGA software or use ClustalX program offline.
this will help you identify the conserved region.
Generate MSF or NEX format output and you can submit to RDP4 program
this will help to identify the possible recombination regions
also mabe this paper help:
http://scialert.net/fulltext/?doi=biotech.2007.93.96
Thanks you all!
Dears, greetings Anyone, please share with me a free plagiarism checker. Thank you
13 March 2023 7,518 1 View
The company provided only one 96 well plate in the kit, which I have used but the reagents in the kit still remains. I am now thinking of using another 96 well plate different from the one the...
11 April 2022 2,646 5 View
Dear RG users, I am dealing with a cervical sample collected with brush and placed in a specimen transport medium (contains 0.05% sodium azide) for HPV detection and genotyping. I have also a...
23 March 2020 1,236 3 View
Dear RG users, I am preparing a protocol for systematic review and meta-analysis of the molecular epidemiology of a particular virus in my setting. Which kind of article quality assessment tool...
20 July 2018 3,537 13 View
I NEED TOPICS ON EMERGING CHALLENGES IN AQUACULTURE
27 June 2018 3,300 21 View
Antimicrobial resistance (AMR) is a global problem to the fight against most infectious disease. Our planet costs a lot because of widespread AMR. Scientists in the field urged that stakeholders...
09 April 2018 4,529 3 View
As we all know , TB is the disease of poverty. We are learning from different reports that the global burden and impact of TB is decreasing from time to time due to a number of interventions....
21 March 2018 4,342 13 View
Reports showed that, since the discovery of some resistant isolates of Neisseria gonorrhoeae against sulfanomide in 1930s, wide spread resistance was also reported in the last 80 years for the...
12 March 2018 5,011 4 View
I have learned from different literature is that the origin of viruses is not well known though there are different theories on the subject. I am looking explanations from RG users on current...
04 March 2018 3,014 4 View
The human microbiota/ normal flora comprises the populations of microbial species that live on or in the human body. The biggest populations of microbe reside in the gastrointestinal tract....
23 February 2018 9,404 8 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...
03 August 2024 3,371 3 View
TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...
03 August 2024 2,484 1 View
I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?
02 August 2024 3,143 4 View