Hybridoma stops producing antibodies after thawing from liquid nitrogen?

Dear scholars, Our research is currently involved with hybridoma technology. We have isolated some original clones after fusion and we are preparing the subclone step to find some true positive...

01 December 2020 1,178 3 View

Does crystallinity degree, (and crystalline phase) of a polymer affect its surface energy and adhesion?

I have several PLLA electrospun nanofibers with different crystallinity degrees and also different crystalline phases and I am measuring the adhesion force by AFM-based techniques. I wonder if we...

28 November 2020 3,269 5 View

Can anyone explain this result?

Recently I used improved Hummer's method to synthesize Graphene Oxide. Then I analysed it with XRD, but beside ~10 degree peak of GO, the was high intensity before the peak that should be straight...

24 February 2019 210 2 View

Help me have subroutine project of DEFORM-3D, which can compile in Absoft Fortran 16.0?

Hi everyone, Now I work with DEFORM-3D (version 10.2) on Windows 7 64bit, I have installed Absoft Fortran 16.0. There is ready project "DEF_SIM_USR64_Absoftv110.atools" in directory of DEFORM...

10 January 2019 3,595 2 View

What is the best solvent for poly ethylene-co-ethyl acrylate (EEA)?

16 January 2018 524 3 View

Asking about primer design for qPCR?

Hi everyone, I have been facing with a very big challenge to design some primers for qPCR. My problem is that I need to design some primers to test the expression of some stressed genes in...

11 September 2017 7,859 3 View

How to determine a mixture has reached a saturation stage yet and their vapor concentrations (in ppm)?

Hello everyone, Suppose that we have a mixture of liquids at a specific temperature. How can we determine it has reached a saturation stage yet? Then, how to calculate the saturation concentration...

11 June 2017 7,148 7 View

Which solvent should I use to dissolve Poly vinyl alcohol-co-ethylene, Poly acrylonitride and Poly(4-vinylpyridine)?

28 June 2016 3,282 3 View

How to accurately capture the load-displacement curve of frame structures at post-collapse (or post-inelastic buckling) regime?

02 March 2016 2,384 3 View

What are giant resonances? What are cluster states?

Giant resonances are collective excited states, high energy and small amplitute

14 April 2014 2,882 3 View

What is Nano technology with coronavirus helpe the human..and is it nano mterals removal of covid19?

I anwar by adsorption or reaction ..but it is either more method...

02 March 2021 4,811 2 View

How to compare different concentrations of nanoparticles for characterization from ICP-MS?

I took measurements of different concentrations of 15 nm and 5 nm Au nano particle solutions. I'm trying to determine which concentration I measured gives the best reading overall. I also...

02 March 2021 7,626 1 View

LEDs on Vis Light photo degradation of MB water sol via N doped TiO2 in a mini flume?

Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...

26 February 2021 4,026 5 View

Zno nano particles colour changed from white to grey in process of calcination any suggestions?

In the formation of Zno nano particles process, after drying at 120 degree C, my zno nano particles are completely white but when i calcine it for 450 degree C it converts into grey colour. i...

26 February 2021 2,435 4 View

What is the best solvent to remove PDMS from nano particles?

I want to remove PDMS oligomers from the surface of nanolayers. I have just read a very helpful paper about the compatibility of PDMS with different solvents( Solvent Compatibility of...

24 February 2021 7,845 5 View

QPCR tubes for Roche Lightcycler Nano?

We own a small cPCR thermal cycler from Roche, Lightcycler Nano, which is convenient for backup quick runs. Recently, Roche has discontinued the plastics for it and they offer no alternative from...

17 February 2021 8,694 5 View

Best synthesis method for large scale silver nanoparticle synthesis with high yield?.Best post processing steps after nanoparticle synthesis?

I have chemically synthesized silver nanoparticle to combat microbes on wound site. I have used many methods of synthesis, but I found minimal field of silver NP product.I am getting whitish...

11 February 2021 4,964 13 View

Dear colleagues. How can we implement the kinetics of the polymerization of aromatic amines in easier and simpler ways?

Dear colleagues. How can we implement the kinetics of the polymerization of aromatic amines in easier and simpler ways? Are there simpler methods for Nano polymers (other than those obtained with...

09 February 2021 5,783 3 View

Concentration of DNA?

Hello everyone! Is a normal value 295.8 ug/ul for cDNA? If not what does it mean? Where is the mistake? The measurement was done with nano drop. Thank you!

09 February 2021 6,202 1 View

How can I calculate volume fraction of MWCNT from a nano hybrid composite ?

I am adding 0.1wt percent MWCNT to glass fiber epoxy composite and want to determine the volume fraction of the MWCNT from this composite but I don't know how to calculate it. can anyone help me...

09 February 2021 8,883 3 View