I want to check expression of certain genes of bacteria. But bacterial preparation with different treatment is taking long time. This is why i want to store bacterial pellet after centrifugation in -70 degree refrigerator and extract it in my available time for expression analysis by real time PCR. I am wondering is there any effect of frozen condition on mRNA or not? Looking for an answer from experienced corner.
You can use Qigen Bacterial protect 1:1 ratio (different than company suggested) or RNA later (Ambion) basically adding 1 mL on your pellet would work, but keep overnight in 4C and place to -20 or -80 both is fine.
I have used both for bacteria GR + and GR - they work OK; and they are easier than -70 because of handling and keeping the same expression profile by using them immediately...
Good luck I hope it helps...
Ali
- Badges
- Science method