How can you optimize the utilization of CRISPR-Cas9 for efficient delivery of mammalian cell lines? What are the key factors to consider?

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What, in your opinion, is the most critical factor to prioritize for high editing efficiency while keeping cytotoxicity low? Could you share any technical hacks that are not commonly known but are useful?

Chinua Onyebuchi

Optimize CRISPR–Cas9 delivery in mammalian cells by selecting the appropriate delivery method (RNP, plasmid, or viral vector), using high-quality guide RNAs, adjusting transfection conditions, and considering cell type, cell cycle stage, and off-target minimization.

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What would you do next?

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Designing Inclusive City. What's your opinion regarding my group's campaign below?

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