Dear Colleagues,
I am writing to consult regarding a recurrent phenotype observed in our transmission electron microscopy (TEM) studies of Phoenix cells with a deletion in the VPS13B gene.
A significant proportion of the knockout cells exhibit abnormal intracellular membrane structures. These appear as relatively straight, often stacked, membrane formations, likely derived from the endoplasmic reticulum (ER). A notable feature is the apparent thickening of these membranes.
Has anyone encountered a similar phenotype in the context of mutations affecting organelle morphology? We would be very grateful for any insights or suggestions regarding the potential underlying mechanisms. TEM VPS13.pdf Thank you in advance for your assistance.
Hello
In images 1 and 3, it looks like your structures are linked to nuclear membrane.
they almost look like inclusions, like 2 cells intersecting but cut at the same level
VPS13B is localized on the ER, ERES-Golgi boundary and the Golgi. Figure 1 and 3 are ER, Figure 2 looks like Golgi.
Article Sec23IP recruits VPS13B/COH1 to ER exit site–Golgi interface...
Studies on your gene and sperm acrosome formation show the golgi and endolysomes, TEM images:
https://pmc.ncbi.nlm.nih.gov/articles/PMC11104845/#:~:text=Abstract,apparatus%2C%20acrosome%2C%20and%20endolysosome.
The increase in membrane thickness results from decrease in membrane fluidity with changing lipid composition resulting from the membrane lipid transporter mutation:
Article Comprehensive Insights into the Cholesterol-Mediated Modulat...
Dear colleagues @Nicolas Barois, @Héctor Osorio-Vega and @Ellen A G Chernoff. Thank you all very much for your attention to this question.
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