Hi.
I had xenografted MDA-MB-231 cell lines to NOD-SCID mouse 46 days ago.
I mixed Matrigel (+Growth factor) with PBS diluted 1x10^7 MDA-MB-231 cells, and xenograft to mammary fat pad orthotopically.
In general, when i had done similar in vivo experiment using MDA-MB-231/468, Tumor growth is okey, but in this time, Tumor volumes are increasing at first 2-3 weeks, and decreased to 40mm^3, and halted almost 3~4 weeks.
Is there any options to grow that tumor?
Can i xenograft again to that mouse in the same site of previously Tumor xenografted?
We have had that happen in our lab as well with Fox Chase SCID beige mice. We usually just remove them from the study group. They will not grow the same tumor but we have had some luck with starting a different tumor type in them.
Good luck!
Did you treat with any therapeutic agent after your tumour inoculation of these mice? How often did you measure tumour volume?
No therapeutic agent given.
We normally measure tumor volume with caliper measurements but that is not accurate if you have an odd size tumor. You can also do BLI imaging to get some sense of size if you have a luc reporter.
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