How to show arrows over the streamlines in CFD-post?

I follow this to make streamlines like the photos attached below: (software- CFD post-processing) First I plot the 2D streamline over a surface "streamline 1". them I plot a vector by the...

01 March 2021 8,668 3 View

How to increase mesh quality of a cfd model in ansys fluent?

How can one improve mesh skewness, orthogonality, aspect ratio?

28 February 2021 7,730 3 View

Why is my CFD problem (mini heat sink rectangular channel) not converging while having good quality mesh?

My CFD problem is having two interconnected rectangular channel, which is known as mini heat sink. I have already performed parallel flow inside of an interconnected mini heat sink. However, I...

19 February 2021 3,209 3 View

Polyelectrolyte complex ion pairing through IR?

Is it possible to observe ion pairing (contact ion or solvent separated ion) of polyelectrolyte complexes through liquid FTIR? Although the PECs have been widely studied in the last two decades, I...

12 November 2020 4,800 1 View

How can I get better picture for the attached TEM Image??

I used TEM equipped with tungsten gun to obtain attached image (several other too, like shown). Side blotting technique followed by negative staining (1% PTA for 30 sec) was used. I cannot get the...

31 October 2020 1,902 5 View

How can I get Nusselt number for mini rectangular channels using fluent?

I am validating my CFD mini rectangular channel model. For that purpose, I need to know how to get the Nu number accurately. For Calculating Nusselt no. from fluent post-processing as follows:...

24 October 2020 3,444 5 View

What is the possible biological explanation of the mutational pressure as a driving force on the evolution of particular species ?

In one analysis major role of mutation pressure was observed in codon usage bias of some species

19 October 2020 4,755 1 View

Do family demographic processes link socioeconomic status to men's physical health?

Previous literature suggests that lower socioeconomic status (SES) men through higher family sociodemographic pressure and lower capacity of stress control, compared to higher SES men, are more...

08 October 2020 2,740 1 View

Is poorer capital at home and at school linking minority children's lower SES to their underachievement?

14 September 2020 8,297 4 View

I am trying to amplify a gene in Arabidopsis with it's promoter. Anyone has tips about the PCR protocol I need to follow?

My product size would be around 5kb with 5' and 3' flanking regions. I have good DNA and primers also worked in other experiments, I was wondering how should I optimize the PCR conditions. Any...

02 September 2020 9,552 4 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

What should be the duration of exposure to simulated space environments with Rodents?

Hi, I am currently working on a pilot study to get some data related to the impact of long duration spaceflight to the microbiome and the immune system. I plan to use rats and simulate...

22 February 2021 592 1 View

HPLC baseline failure (ACN/Water)?

My baseline increases without any explanation, it is only fixed by changing the solution B, will it be the column? Reagents? Bomb? Detector? Solution A: H3PO4 0.01% IN WATER Solution B: H3PO4...

21 February 2021 5,656 3 View

Which transfection reagent is the best for plasmid transfection of MDA-MB-231 cell line, Lipofectamine based or other reagent suggestion?

I try to transfect MDA MB 231 cell line with a plasmid by PEI, unfortunately, the rate of transfection is low, and now I wanna find and order the best transfection reagent for this cell line and...

19 February 2021 828 6 View

Any information about dinitrosalisylic acid reagent end point color stability ?

I work on estimation of alpha amylase activity using dinitrosalisylic acid reagent If i put the reagent to stope the enzymatic reaction and allow the reagent to react with the produced reducing...

17 February 2021 7,402 4 View

Has anyone performed a colorimetric RT-LAMP assay using Lucigens LavaLAMP kit?

Hi there, my lab recently opted to use LavaLAMP from Lucigen instead of the Warmstart kit from NEB. However, Lucigen has yet to create a colormetric kit for RT-LAMP, and adding phenol-Red (pH...

17 February 2021 5,399 1 View

How can you tell if your organotypic slice culture is healthy ?

I'm working on organotypic cerebellar slice cultures from postnatal rats and I'm not sure how to tell if the slices are healthy at any given point. I know there should be a lot of microglial...

15 February 2021 2,830 2 View

Why daily dexamethasone given to rats fed high fat/fructose/salt cause very high mortality rate?

rats were given daily dexamethasone in drinking water corresponding to 0.5 mg/kg or 2 mg/kg. These rats are simultaneously fed 25% fat, 3% salt in diet and 10% fructose in drinking water but...

15 February 2021 7,634 2 View