We are searching for the best accessible vein, and an approach without anaesthesia.
You may try external jugular veins. And you can search "scalp needle" "butterfly needle" or winged needle for better controling the iv.
Almost certainly you would need a needle on a tubing for this and an assistant - so you could scruff the pup and hold the needle and someone else operating the plunger - if you can use a butterfly needle that might be best - however I think 25G might be the smallest available though and this might be too large for you in P11 pups.
For P0-P2 injections we have used a 32G or 34G Hamilton needle and some Polyethylene tubing and Epoxy resin to make a very similar construct and place it on a larger blunt needle and syringe; however these are not ideal for repeated sterilisation as they are quite fragile. The skin may also be too tough for the small gauge.
No sure if Simon N Waddington has any suggestions about where to access the vessel in this age?
Speak to Dany & Sonam in Julien B's group - they are highly skilled
Thanks, everyone! Yung-Chieh Lin Joseph Davidson Simon N Waddington
Highly appreciated!
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